Isolation And Identification Of Pathogens Of Bovine Endometritis In Xinjiang And Selection Of Herbs For Its Treatment

Endometritis that is caused by the bateria infection has been disease frequently occurred during the early postpartum period in dairy cattle, which can result in reproduction obstruction.The aim of the study was to isolate and identify the pathogens that lead to endometritis of dairy cattle in Xinjiang, then the sensitivity test of the pathogens to antimicrobials was conducted. The traditional chinese herbs with available therapeutic potential were screened by means of the tests of bacteria inhibition in vivo and in vitro and the motility of the uterus in vitro so as to compose the oral or intrauterine herb compounds for the therapy. Uterine secretion was collected aseptically from 25 infected dairy cattle in xinjiang. And the pathogens were identified by microscopy, cultivation of the selective or differential medium and the micro-biochemical method. The isolated bacteria included B.cereus(24.5%), non-haemolytic streptococcus(18.4%), E.coli(14.3%), haemolytic streptococcus (10.2%), S.aureus (10.2%), S. epidermidis (10.2%), C.fetus (10.2%) and Acinetobacter (2.0%). G+ bacteria were 73.5 % among the bacteria isolated. While the 47.6%, 28.6% and 23.8% of dairy cattle were infected by one, two and three species of the bacteria, respectively, showing that the ratio of mono-bacterial infection was higher than that of the mixed bacterial infection. The sensitivity of the isolated bacteria to 13 kinds of antimicrobials was determined by disc agar diffusion, most of which were sensitive to kanamycin, gentamicin and quinolones. 19 herbs which were extracted with water separately were used to investigate their inhibiting function to each species of isolated bacteria by the broth microdilution method. The results showed that Rhubarb , Weeping Forsythia Capsule and Honeysuckle had high inhibition in vitro against most of the isolated bacteria. Additionally, the minimal inhibition concentration (MIC) values of intrauterine infusion compounds 1, 2 (U1 and U2 ), oral compounds 1, 2 (O1 and O2) to those isolated bacteria were tested using aforementioned method ,and showed that U1 was the most sensitive to the isolated bacteria among 4 herb compounds. The concentration of the bacteria was minimal in 12 hours fallowing uterine inoculation of the E.coli mixed with either U1 or U2 and S.aureus mixed with either U1 or U2 in rat, from that time on, the concentration of different bacteria in the uterus was increased gradually. Morever, compound U1 was more effective in inhibiting the isolated E.coli or S.aureus than other 3 compounds in vivo. The exciting effect of 25 herbs extracted with water and 4 compounds on uterine smooth muscle was tested in vitro in rat by transducers coupled with the biomedical signal recording system. Carthamus tinctorius and Herba dianihi were more effective than other herbs. Whereas compound U1 induced more powerful contraction in comparison with other 3 compounds,and the dose-denpendent manner was presented between 0.1% to 0.8% of concentration in U1 and U2. However, the curve of contraction tended to be constant when the concentration of compounds was higher than 0.8%. Edometritis model in rat was developed by the stimulation with 5%NH3.H2O or 3%acetic acid for 3 days ,then infecting the mixture of the isolated E.coli and S.aureus. This model was helpful for the investigation of pathogenesis and therapeutics of endometritis.