Studies On Phytoremediation Of Heavy Metal-contaminated Soil In GAP Bases Of Ligusticum Chuanxiong Hort. And Tissue Culture Of The Herb

Ligusticum chuanxiong Hort. is one kind of famous traditional Chinese crud drugs for curing many illnesses in Sichuan Province. With the gradually increased environmental contamination, such as As, Hg etc, the concentrations of the heavy metals in Ligusticum chuanxiong Hort.were excessed to the standard qualification for the poisonous elements accumulating in the plant of herbs. The export of the crud drug of Ligusticum chuanxiong Hort. was obtstacled in recent years. Therefore, studies on how to reduce the heavy metals in crude drug is of great imoportance.The aims of the thesis were to deal with tissue culture of Ligusticum chuanxiong Hort and phytoremediation of polluted soils in GAP bases of Ligusticum chuanxiong Hort. Tissue culture is performed to bring breedlings of Ligusticum chuanxiong Hort with a character of less heavy metals than those planted by conventional agriculture. The phytoremediation will decrease the concentrations of the heavy metals in soils of GAP bases, which is a main source of heavy metals in the plants of Ligusticum chuanxiong Hort.Fresh shoots of Ligusticum chuanxiong Hort were used as explants in the culture tissue. The different harmone proportions with 6-BA and 2,4-D as hormones to induce callus and 6-BA, IAA to induce buds differentiating were comparison in detail. It is discovered that the addition of 6-BA (0.4mg/L) and 2,4-D(2.0mg/L) in MS culture metrix is suitable to induce callus with the callus ratio of 87.5%, while addition of 6-BA(0.4mg/L) and IAA(0.5mg/L) is propor to induce buds but with low bud differentiating ratio of 40%, so more work needed to find out the best proportion of harmones for the later one.Nephrolepis auriculata (L.) Triman and Glycine max (L.) Merr. collected from the GAP bases of Ligusticum chuanxiong Hort are investigated as the effective hyperaccumulators. The concentrations of As, Hg in four tissues (seeds, roots, shoots, and leaves) were measured. The distributive behavours of Hg and As were obtained in details. It is discovered that the concentrations of arsenic and mercury are the highest in the leaves whereas the least in the seeds among the four partions of the kinds of phytoaccumulators for heavy metals. Those reseaches demonstrated that plant of Nephrolepis auriculata (L.) Triman is good hyperaccumulator for Hg and As, and however Glycine max (L.) is with a character of hyperaccumulator for Hg only, respectively.