Studies On Molecular Polymorphisms Of H-FABP And CAST Genes And Their Relarionships With Meat Quality Traits In Pigs

The study was mainly performed using 242 (slaughter 27) Large white and 159 (slaughter 38) zhongxu Black pigs on swines. The genetic variations of H-FABP gene and CAST gene were detected by PCR-RFLP. Twelve meat quality traits of slautered pigs were measured and analysed. Furthermore, relation of the genotypes including H-FABP and CAST with meat quality were investigated respectly, Results showed as follows:1 , There are polymorphims in all site in H-FABP gene in two breeds. The frequencies of H allele of HinfI site in Large White and zhongxu Black were 0.7273 > 0.8578 respectively; The frequencies of D allele were 0.7480 and 0.5881 respectively of HaeIII-RFLP polymorphic site; The frequencies of B allele were 0.5889 and 0.5943 respectively of Hinfl*- RFLP polymorphic site. In the CAST gene, The frequencies of C allele of Mspl site in Large White and zhongxu Black were 0.6921 and 0.5283 respectively; The frequencies of F allele were0.5889 and 0.5943 respectively of HinfI-RFLP polymorphic site.2, H-FABP gene(l)The Hinfl-RFLP significantly influenced on IMF of Large white; The Hinfl -RFLP significantly influenced on Marbling of Large white.(2) The Hinfl- RFLP significantly influenced on SFV of zhongxu Black;(3) Being on whole experimental pigs: The Hinfl - RFLP significantly influenced on IMF; The haploidy of hh-DD-BB has hingest IMF, and Hh-DD-BB has least SFV.3, CAST gene(1) The MspI - RFLP significantly influenced on SFV of Large white.(2) Being on whole experimental pigs: The MspI- RFLP significantly influenced on IMF, The haploidy of DD-FF have the hingest IMF and the least SFV.4,The correlation amongst meat quality traitsMarbling has positive correlation with IMF and PH_U significantly (P<0.01) ; Marbling has negtive correlation with SFV significantly (P<0.01) ; IMF has negtive correlation with SFV signify-cantly (P<0.05) and WM (P<0.01) ; SFV has negtive correlation with pH₁ significantly (P<0.05) .As a whole, this study provided an elementary foundation the for investigating whether H-FABP locus is the candidate gene for IMF deposition and CAST locus is the candidate gene for tendrness in Large white and zhongxu Black.