Study On Rumen Ferment Of Different Levels Of Molybdenum & Copper & Nitrogen & Sulfur And Effect On Metabolism Of Nutrients Of Rumen

The experiments studied the effect on the rumen fermentation and fiber degradation under different feeding levels of nitrogen, sulfur, molybdenum, or copper with dual -flow continuous culture system(DFCCS-â…¢) and rumen nylon-bag method.The optimum additive levels of copper and sulfur with the diet of different levels of nitrogen, molybdenum were also gained.Crossbred (Limousin × Lu-xi) beef cattle, fitted with permanent cannulas in rumen were used . Metabolism of nutrients was investigated by the determination of the disappearance rates of NDF and ADF and bacterial nitrogen. Rumen fermentation parameter (pH ,NH₃-N concentration ) were also measured.The test grounded on some invivo research findings via regression analysis,further demonstrated the scientificaalness of DFCCS- â…¢This experiment accessed the effects of dietary NPN, sulfur and copper on artifical rumen fermentation. The results indicated that with the ration of urea increasing, appending inorganic sulfur can significantly improve rumen fermentation efficiency(P<0.05), increase the disappearance rate of ADF and NDF ,improve the digestibility of cellulose. Supplement of sulfur to diets with urea significantly improved the availability of NPN (P< 0.05). While the nitrogen to sulfur ratio was the same, the disappearance rates of NDF and ADF in artificial rumen were increased with the increasing dietary copper (P< 0.05).The test also studied the effects on the rumen fermentation and fiber degradability under different feeding level of molybdenum and copper with DFCCS- â…¡. The result showed that the copper supplemented had significant effects on the disappearance rate of ADF and NDF and increased bacteria nitrogen synthesizes at the same molybdenum appended level(P < 0.05). But it had insignificant effects on the concentration of NH₃-N and pH (P >0.05) .It suggested that the optimal requirements of S were 0.150%, 0.225%, 0.300% for DFCCS-â…¢ fed diets containing 0%, 1%, and 2% urea respectively; the optimal requirements of copper were 10, 10 and 25 mg/kg for DFCCS-â…¢ fed diets cntaining urea and sulfur 0% and 0.150%, 1% and 0.225% , 2% and 0.300% respectively.The optimal requirements of copperBy in vivo and in vitro nylon-bag methods, the experiment assessed the degradation of NDF and ADF of four roughage.By regression analysis ,we could found that NDF and ADF has corking relativity. The regression equations for estimating the degradation of NDF and ADF assessed by in vivo from which measured by vitro were established as follows:The degradation of NDF:Wheat bean y = 0.9762x + 4.3289Corn stalk y = 0.9392x + 5.3947Sweet potato stalk y = 0.9478x + 4.2911Peanut vine y = 1.0273x + 1.2341The degradation of ADF:Wheat bean y = 0.8582x + 6.4986Corn stalk y = 0.9215x + 5.822Sweet potato stalk y = 0.7566x + 8.0836Peanut vine y - 1.0795x + 0.245y: The degradation measured by in vivo(%);x: The degradatio nmeasured by in vitro(%) <,R2 = 0.9862n=8R2 = 0.9927n=8R2 = 0.9469n=8R2 = 0.9856n=8R2 = 0.8344n=8R2 = 0.9853n=8R2 = 0.8528n=8R2 = 0.8949n=8...