Studies On Anti-inflammatory Effects And Its Mechanism Of Taurocholic Acid

Taurocholic Acid (TCA) is a kind of tauro-conjugated bile acid which exists in many kinds of animals' bile. It belongs to conjugated bile acid. TCA were abs-tracted from bovine bile by using simple biochemistry technology such as salting-out,extraction,dehydration;separated by silica gel.The TCA were measured by thin layer scanning and absorption spectrometer, and its purity were more 97%.and itsthan recovery rate is 61%. In this project adopts the improved karber measure todetermine the acute oral toxicity of TCA, the LD50 is 4400mg/kg . The contents of inflammatory media in tissues were measured by using Fluor-o-spectrophotometry and UV-spectrophotometry for studying the anti-inflammatory mechanisms of TCA. The results showed that TCA could significantly reduce the contents of inflammatory media (Such as NO,PGE2,Histamine) in inflammatory tis-sues of rats. Both high and low dosage of TCA could obviously inhibit the infla-mmation and the content PGE2 which were induced by the methods of Auricular concha swelling,fitering out piment,cotton ball granular swelling and feet swelling.The content of the communication molecule-nitric oxide (NO) and the Histamine were also tested,and the mechanism of these action were disussed. TCA could ob-viously decreased the level of in Histamine blood in rats,but it could obviously r-edused the level of NO in brain tissue and plasma in rats.