The Analysis On Growth Capability And Application On Parentage Identification By The Microsatellite Markers Of Sheep

The polymorphism of 10 microsatellites in four meat sheep breeds were analysed in this paper. Allele frequencies, effective number of alleles, heterozygosity, polymorphism information content, genetic distance were calculated for all microsatellites in each groups and phylogenetic was analysed as well. A total of 107 alleles were detected from ten microsatellite loci and the number of alleles observed at a locus varied between five and thirteen. All microsatellite loci were highly polymorphic, with the PIC ranging from 0.4985 to 0.8904. All populations showed high levels of heterozygosity with the lowest value of 0.5630 and the highest value of 0.8995. The genetic distance were far in four meat sheep. The Suffolk and Dorset sheep were first grouped together with the nearest genetic distance of 0.34525, and then together with Charolais, finally together with Chinese Merino. The genetic distance between Chinese Merino with Charolais was the farest of 0.51639. The results may be useful as an initial guide in evaluating the level of genetic variation, developing conservation strategies and predicting heterosis.The amplified fragment length polymorphism of ten linkage microsatellite DNA loci in four and six chrosome according to the sheep linkage map and reports were detected in 162 meat sheep of Chinese Merino. By the multicomarison for weight of different genotypes in ten microsatellite loci and F test for the relativity of microsatellite markers with weight traits, the results showed that close linkage OARHH35 and BMS648 had a significant difference in weigh (P<0.05). There is weight QTL and probably ob gene . BM9058(131/149) , BM4621(149/181) , BM4311(119/119), OARJMP8(131/145) , OARHH35(123/155)and BMS648(176/208)had positive correlation with weight.Ampliations of nine micosatellite loci on nine Dorset sheep were performed by aPCR method. The mixed products of amplification by three microsatellite DNA were separated in polyacrylamide gel and analyzed with the sliver staining. The paternity was calculated by 99.997% on the basis of ear samples from two Dorset sheep with confirmed paternity and seven with unknown paternity. The results showed that the microsatellite DNA technology for parentage identification in sheep with excellency of sensitivity stabilization and nicety.