Studies On Tissue Culture And Somatic Embryogenesis Of Parthenocissus Tricuspidata And P.quinquefolia

Parthenocissus tricuspidata and P. quinquefolia are plants of Vitaceae. They are good vertical greening plants in china. Tissue culture and somatic embryogenesis of P.tricuspidata and P. quinquefolia were mainly studied. All the results are beneficial character of cell engineering of Parthenocissus plants. The results indicate as follow:(1) In P.quinquefolia tissue culture, the best medium of seed explants is B5 or 1/2MS without PGR; The optimal medium of bud multiplication of subculture of P. quinquefolia is B5 + BA 0.5mg/L + IBA 0.05 (or 0.1mg/L) + sugar 20 g/L + agar 5 g/L (Sugar and agar contents are same in follow medium) or B5 + BA 0.5 mg/L + NAA 0.02 mg/L; Rooting medium of P. quinquefolia is B5 + IBA 0.1 mg/L. After acclimatization the survival rate of transplantation is over 90 %.(2) The experiments show that shoot tips are the suitable explants for tissue culture of P.tricuspidata(endemic species). In initiated culture, B5 is the best basal medium and the optimum BA concentration is 0.3 mg/L. The suitable culture medium for bud multiplication of shoots is B5 + BA 1.0 (or 1.5 mg/L) + IBA 0.1 mg/L or B5 + BA 1.0 (or 1.5 mg/L) + NAA 0.05 mg/L. The shoot rooting medium is B5 + IBA 0.2 mg/L. The rooted plantlets are transplanted into the pots containing 1/2 vermiculite +1/2 perlite. The survival rate is over 93 %.By experimental comparisons the imported species has more serious browning than the endemic species in tissue culture. Adding VC is more effective method to lighten browning than PVP and AC. The suitable Vc concentration is 1.5 g/L and the browning rate is only 25 %.(3) Somatic embryogenesis from P. tricuspidata was studied. The results show thatsomatic embryos are obtained from cotyledons of P. tricuspidata which are induced on MS medium containing 0.3 mg/L BA and 0.1 mg/L NAA or 1/2MS medium containing 0.5 mg/L BA and 0.1 mg/L NAA, and from cotyledon petioles which are induced on 1/2MS medium containing 0.3 mg/L BA. The somatic embryos induction rate is very low. The highest induction rate is only 18.8 %. Adding CH into the medium and dark culture have no effects on somatic embryogenesis of P.tricuspidata.P.quinquefolia have no somatic embryogenesis. The results confirm that different genotypes have large difference in their somatic embryogenesis.