Variations In Partial Region Of Porcine POU1F1 Gene And Its Relation With Growth Porformance

This research was conducted to analyse the sequence variations in partial region of pig POU1F1 gene, including exon4, exon5, exon6 and intron3, by using PCR-RFLP, PCR-SSCP and sequencing techneque. Six pig breeds (Landrace, Duroc, Yorkshire, Jiangquhai, Meishan and Xiang) were selected as experimental animal. Also a commercial pig population growth performance was correlated with the polymorphism of intron3. The results were shown as follows:1. No polymorphism was found in exonS and exon6 of POU1F1 gene in all breeds. A point mutation but amino acid substitute was identified in exon4. Allele (G, H) frequencies in this restriction site in different pig breeds distributed significantly, with high H allele frequency in foreign meat pigs and low in Chinese local breeds. While among human, pig, mouse and cow, POUHD and POUso encoded by exon4 were high conservation.2. A Msp I polymorphism reported previously was identified in intron3. There was a significant difference of allele frequencies in this site among different breeds as well. The D allele frequency was high in foreign meat pigs, while low in Chinese local fat pigs.3. According to analysis of correlation between growth performance and genotypes in commercial pigs, body weight (expect birth weight) and daily gain in different stages between DD genotype and other genotypes differed significantly (P<0.05) or extremely significantly (P<0.01), respectively.4. By comparing homologous analysis of nucleotide sequences encoding POU domain of POU1F1 protein and amino acid sequences in different species respectively, thehomologous rate between human and porcine POU domain of POU1F1 protein was significantly higher than that of human and other animals POU domain of POU1F1 protein, so pigs can be used to establish animal models of related human diseases, providing scientific foundation and serving for human medicine.