Studies On Microorganism Degradation Of Non-Starch Polysaccharide In Feed

In this paper, three strains (coded 3.316, 3.4265, 5.132, the same to the following) with high-producing B-glucanase , carboxymethyl cellulase and xylanasc were screened from our lab conserving ten strains ,which regarded the enzyme activity as the only index. The optimal culture conditions and methods of every strain were selected after optimazating enzyme-producing conditions, and their enzyme's action character were studied. Evaluated the microoganism degradation result of non-starch polysaccharide as the variation of pentosan and fluid extract viscosity in the feed material for the index. In addition , the action results of their enzyme preparation were assessed with the digestion test in vitro. The results were as follows:(1)Thc optimal carbon source of strain 3.316 was a -lactose with concentration of 4%, the optimal nitrogen source was beef extract with concentration of 0.1%. Cultured 72h at temperature 25C, the B -glucanase activity of strain 3.316 was 736IU/ml .The optimal buffer for B -glucanase was citric acid buffer, the optimal pH was 3.0 , the optimal reaction temperature was 60C, the cellulase activity of strain 3.316 was 830IU/ml .The viscosity of feed material reduced 56.59%(P<0.01), the content of pentosan reduced 3.28%(P>.05) after fermentating. Added the strain's NSP compound enzyme to wheat ,the digestion ratio in vitro of dry material ,coarse fat ,coarse protein, coarse fiber raised 8.08%(P<0.05), 6.72%(P<0.05), 10.66%(P<0.01) and 17.01 %(P<0.05) respectively. The filtrate viscosity of digestion in vitro reduced 2.49%(P>0.05). (2)The optimal carbon source of strain 3.4265 was wheat bran with concentration of 4%, the optimal nitrogen source was ammonium citrate dibasic withconcentration of 0.1%. Cultured 84h at temperature 28C,the xylanase activity of strain 3.4265 was 445.73IU/ml .The optimal buffer for xylanase was sodium phosphate dibasic-citric acid buffer, the optimal pH was 5.4 , the optimal reaction temperature was 50C, the 3 -glucanase and cellulase activity of strain 3.4265 were also high .The viscosity of feed material reduced 67.91 %(P<0.01), the content of pentosan reduced 2.72%(P>.05) after fermentating. Added the strain's NSP compound enzyme to the wheat, the digestion ratio in vitro of dry material ,coarse fat ,coarsc protein, coarse fiber raised ll.l%(P<0.01), 15.47%(P<0.01), 14.70%(P<0.01) and 21.70%(P<0.05) respectively. The filtrate viscosity of digestion in vitro reduced 3.95%(P>0.05).(3)Thc optimal carbon source of strain 5.132 was wheat bran with concentration of 4%, the optimal nitrogen source was yeast extract with concentration of 0.1%. Cultured 216h at temperature 25-28C, the xylanase activity of strain 5.132 was 470.45IU/ml .The optimal buffer for xylanase was citric acid buffer, the optimal pH was 5.0 , the optimal reaction temperature was 50C, the B-glucanase and cellulose activity of strain 5.132were low .The viscosity of feed material reduced 65.31%(P<0.01), the content of pentosan reduced 4.72%(P>.05) after fermentating. Added the strain's NSP compound enzyme to the wheat, the digestion ratio in vitro of dry material ,coarse fat ,coarse protein, coarse fiber raised 6.1%(P<0.01), 15.96%(P<0.01), 11.67%(P<0.01) and 19.29%(P<0.05) respectively. The filtrate viscosity of digestion in vitro reduced 4.90%(P>0.05).