Establishment And Identification Of Hybridoma Cell Lines Secreting Monoclonal Antibodies Against Porcine Reproductive And Respiratory Syndrome Virus

Balb/c mice were immunized with the purified PRRSV - BJ isolate. mouse spleen cell were fused with myelomas NSo .culture supernatants of hybridism's were screed by indirect ELISA. Positive colonies were cloned three times by limited dilution. One cell line named 6PR, which could secret McAb stably was obtained. The isotype of the McAb was IgG1 .the average number of chromosomes of the hybridoma cell line was 99. The ELISA titer of cell supernatant and ascite were 1:256 and 1:20480. The McAb proved to react with NP protein in western-blotting. The McAb did not cross react with PRV. HCV. PPV, demonstrate good specificity .The 6PR cell line could steadily secreted McAb after sub cultured twenty times. The obtainment of this McAb established a foundation of the researches to PRRSV and quick-diagnosis methods.