Studies On Histomoniasis Of Turkeys And Cultivation In In-vitro Of Histomonas Mekeagrides

Clinical pathology and hislopalhology of histomoniasis in turkeys were studied by infecting bronzy turkeys with mature eggs of Heterakis gallinarum systemically. We obtained histomonas meleagridis from the caecum of turkeys effected histomoniasis and cultivated histomonas mcleagridis in in-vitro. The results are as follows:1. The primary clinical symptoms of histomoniasis of turkeys were that body temperature hoisted, spirit depressed, appetite reduced, feather dishevelmented ,wing and offal drooped. At the beginning, feces was watery and had brick red slime, then feces was sulfureous or brown, some times had white lithate .As for serious cases, there was blood in the feces .At last turkeys died of emaciation and exhaustion. The cause of disease lasted a week or so.2. The results of histological pathology showed that changes of turkey in anatomy were mucous membrane of caecum became thicker and caecum became bigger and it looked like banana, some caecum took on necrotic and haemorrhaged cecitis; The liver swelled and had diagnostic necrotic kitchen range. Some were exiguous, offwhite and necrotic point; some were necrotic kitchen range looked like chrysanthemum. The traits of histological changes were that there were cosinophils soakaged at local part of lamina propria, mucous of caecum, lymph follicle accretioned, mucous layer became thicker distinctly, the epithelial layer putrescenced and desquamated widely, the inhere layer destroyed and haemorrhaged; There were many acidophil and indulged in leucocyte collecting in the center of vein of liver. Partial liver cell resulted in fat denaturalized. There were specific necrolic nodes in parenchyma of liver.3.The clinical pathology revealed that quantities of RBC and content of Hb reduced. In serum enzyme, the content of TP ALB TCH and the activity of LDH and ACP reduced, the activity of AST and r-GT hoisted ,but the changes in activity of ALP and ALT were inconspicuous.4.1n the 4 culture media, as for the living time of histomonas meleagridis, culture medium III culture mediumIV culture medium I culture medium II. When it came to multiplication, culture medium III culture mediumIV culture medium I culture medium II.The results show that after turkeys infected histomoniasis, there were marked pathological changes; The content of Hb, RBC reduced in blood and the content of TP, ALB toboggmed,the activity of AST r-GT increased in serum, which were acted as diagnostic indexes; In in-vitro Histomonas meieagridis couldn't survive for long time. If the conditions controlled strictly, we can obtain perfect results.