Studies On The Rapid Propagation Of Chinese Orchid By Tissue Culture And Its Application

Chinese orchids are part of the terrestrial orchids of Orchidaceae Cymbidum, for example C. goeringii, C. faberi, C. ensifolium, C. sinense, C. kanran .The Chinese orchids are respectfully called by the Chinese "one of the four gentlemen" or "one of the four Refined Tastes". Appreciating the orchids , the Orientals attach great importance to such tastes of aesthetic appreciation as purity and freshness, grace and delicacy. Chinese orchids propagation is accomplished by off shoots from the mother plant and slow-growing. So we try to rapid propagation Chinese orchids through tissue culture.The optimal culture conditions of the growth of C. goeringii rhizomes from shoot-tip were studied.The results showed that the percentage of initiated shoot-tip and successful inducing were the highest when those shoot-tips were from the buds 2.5~4cm long. The 1/2MS medium added with 2~5mg/L NAA and 10% CM was useful to rhizomes growth from shoot-tips, while 2~3mg/L BA was suitable for buds differentiation from rhizomes. Favorable effect of 1-2 mg/L NAA and3 g/L AC on rooting culture was observed. The plantlets formed 3-4 leaves and 6~7cm high, they were transferred into pots.Rhizomes were used as simplify experiment materials, in order to cut down tissue culture cost.The results showed that used solid medium and replaced sucrose by sugar, added with 30g/L suger and 3g/L AC for rhizomes proliferation were better than others, the rate of rhizomes forming being as high as908.33%, it could reduced cost to used nature light.Liquid medium and 30g/l sugar were suitable for bud differentiation, but affect of AC was opposite .The rhizomes grew gradually into plantlets that were a few , 30 g/L sugar and 3 g/L AC were better than others.Application and extension the project of the rapid propagation of Chinese orchids by tissue culture , the project feasibility studies were carried out through construct in scale, choose side before construct , plan of construct, business managemeat , investment budget, progress plan and financial analysis. Rapid propagation base couppies10 areas.The project raise yield of Chinese orchids through combine rapid propagation of tissue culture with cultivation techniques of greenhouse. The project explication will centre on layout and installation of tissue culture room, investment budget of fixed assets and circulating assets. The results showed that 2 millions plantlet were cultured every year , project make an investment of 8.48 millions yuan. Investment was taken back for three years. Every year the project makes a profile about 10 millions yuan from third year. The project is the high science and technology content of example project, the project construct take a shortcut that is built of the centre of rapid propagation of tissue culture on the basis of the Chinese orchids nursery.