| The incidence of single petal tuberose virus disease is about 45.95% ,which being cultured in Heilongjiang province.This is lower.But the incidence of double petal tuberose virus disease is higher about 97.3%.Almost all the infected plant have symptoms like mosaic leaf or yellow streak leaf. When floral axis growed we can observed obviouse streak symptoms in it.Virus existing in tuberose make the plant growthing slowly,reducing the number of inflorescence and the rate of flower production,cutting down the time of flowering.And the diseasing flower is small,the color is light.The value of tuberose commodty reduce greatly.The range of tuberose hoest is very narrow. Inoculation 5 family 7species indicating plant against tuberose virus disease show that all the plant were not being infected.The virus maybe only infect tuberose.The content of granular virus is higher,which is existing in floral axis ,leaf sheath, new blade and bud scale.But in buib is lower.Electron microscopic observations of the naturally infected Tuberise revealed that there are fiexuous filamentous particles 750nm.Typical pinwheel type ,bundle are found in the cytoplasm of the mesophyll cell could be observed by ultrathin section of leaves.So tuberose virus can be concluded tentatively that is a kind of potyvirus and about belonging to the second group basing on above conclusion.The concret classify of tuberose virus in Heilongjiang province need to be identified continually.Virus-free plants of tuberose were obtained from in vitro meristem tip apex tissue culture with the basical medium of MS.Each of medium was added 3.0%sucrose and 0.8%agar and the pH value is 5.7-5.8.A series of optimization experiment for cultural medium composition,concentration of phytohormones were investgated with a view to acclerate the propagation of virus 0 free plants.The result of virus elimination by meristem tip culture of purpie-skined tuberose commonly grown in North-east agriculture showed that the best medium shooting was MS+ LAA0.1 mg/1 +2-ip0.3 mg/L,the rate of taking shoot is 100%. And that for rooting was 1/2MS+IBA1.0 mg/L +NAA0.01 mg/L, the rate of taking root is 100%.Effect of virus-free culture with different meristem tips in size and heated -treated aer discussed. The result showed that pretreatment of cloces with 80 thermotheray for 10min,that obtained virus-free plants by using 0.3-0.5mm meristem tip.This paper deals with studies on in vitro culture of meristem tip of Tuberose .including callus induction and differetiation, plantlet root development and transplantation. Resultsshowed :It was advantageous to callus induction on MS+2,4-D1.5 mg/1 +BA0.5 mg/L,the frequency of callus induction was 90%.MS+ LAA0.1 mg/L +2-ip0.3 mg/Lwas the best medium for callus differentition of which ratio was 87%,the maen number of plantlets per callus was 6.7.The best rooting medium was 1/2MS+IBA1.0 mg/L +NAA0.01 mg/L, 100%of the plantlets could develop their root.After the regenerated plantlets were transplanted to soil,they could adapt to environment.The survival ratio of transplanting reached 100%.The researching of plantlet bulbing of Tuberose showed :It was advantageous to plantlet bulbing on the test tube of 4~6cm high,and pH6.8 , sucrose90g/L concentration of PP33310mg/L. Mean bulblet diameter was 5-10 mm made the ratio was 97~100%.Bulb with leaves and roots can make the ratio higher.From these results,an effective procedure for mass propagation of virus-free tuberose by tip culture was established. |
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