Interaction Between Rootstock And Scion Of The Grafted Hevea Brasiliensis In Physiological And Biochemical Characteristics Of Latex

In natural rubber production, the planting materials of llcvca hrusilicnsis are the high yeilding clones budded on seedling rootstocks. Study of interaction between rootstock and scion of the budded trees is important for the selection of optimum rootstock. In the present study, by using latex diagnoses, PAGH and SDS-PAGL, the physiological and biochemical interaction \vas investigated with the sell-root clones Reyan88-13 and Haiken-2 that were self- and inter- grafted. Results were summarized as follows.1. There was interaction in the physiological characteristics of latex between the ruotstock and scion of the grafted trees. The effect was caused mainly by rootstock on dry rubber content, inorganic phosphate content and magnesium content of the latex. on sucrose content mainly by scion, and on thiol content by rootstock and scion. Besides, the interaction varied with different combinations of rootstocks and scions from separated clones.2. The interaction in the physiological characteristics of latex between rootstock and scion changed along with prolonged latex exploitation. The change of interaction was different between combinations of rootstock and scion. For scion, the change was related not only to the clones of scion but to the physiological characteristics. For rootstock. as smaller influence was found from the scion, the changes in the physiological characteristics were similar between the combinations of rootstock and scion. In addition, the interaction changed under latex exploitation with ethrel stimulation.3. Latex revcrtase was studied. There was no obvious interaction in the activities of latex reverlase between rootstock and scion, which kept their characteristics respeclively.4. There existed significant and comparatively steady interaction in C zone of the esterase isozyme zymogram of the latex between rootstock and scion. This interaction was not obviously affected by latex exploitation with or without ethrel stimulation. It was deduced that latex movement in laticiferous ducts caused by latex exploitation was one of the reasons for the interaction between rootstock and scion.5. There was no significant difference in the peroxidase isozyme zymogram and the soluble protein electrophoretogram of latex C-serum between clones Reyan88-13 and Haiken-2. and there was also no obvious difference in those zymograms between rootstock and scion of the self-grafted rubber trees. It showed that the peroxidase isozyme zymogram and the soluble protein electrophoretogram of latex C-serum could not clarify the interaction between the rootstock and scion of grafted rubber tree.