Study On The Technique System Of Micropropagation In Cymbidium Grandiflorium

The shoots of Cymbidium grandiflorium were used as explants to establish a rapid technique system of micropropagation, and to find out effective measures to prevent the browning of shoot explants. The results were as follows:1) Temperature for culture: Day 25℃, Night 23℃. 14 hours illumination every day, and about 2000 Lux illumination intensity. The pH of medium was adjusted to 5.3-5.6.2)The best initiation medium was MS+BA1.0mg/L+NAA0.01mg/L+AC0.5g/L. After 25 days culture, the rate of initiation could reach 85.7%.3)The multiplication of shoot clumps:MS+BA2.0mg/L+NAA0.1mg/L+AC0.5g/L was the best medium. After 80 days culture, the multiplication rate of shoot clumps could reach 6.23.4) The inducement medium of the PLB: l/2MS+BA1.0mg/L+NAA0.1mg/L+ ACO.lg/L. After 80 days culture, the inducing rate of the PLB could reach 71.4%.5) The multiplication of the PLB:l/2MS+BA1.0mg/L+NAA0.05mg/L+AC0.1g/L was the best medium. After 90 days culture, the multiplication rate of the PLB could reach 50.6) Seedling stage: the medium for the elongation of shoots and leaves was l/2MS+BA2.0mg/L+NAA0.1mg/L+AC0.5g/L. the medium for the rooting of shoots was 1/2MS+BA1.0mg/L+NAA0.1mg/L+AC0.5g/L.7) Active carbon was effective for preventing the explants from browning to a certain extent, 0.5g/L was the best initiation concentration, 0.1 g/L was the best inducement and multiplication the PLB concentration.8) For nursling of plantlets, the soil of the rotten leaves in the bamboo groves was the best material, the survial rate of plantlets could be up to 100%, after transplanting soil.