Preliminary Studies On Foreign DNA Delivery To Penaeus Chinensis With Gene Gun And Electroporator

Transgenic Chinese shrimps were originally obtained by gene gun and electroporator. A plasmid pGDNA-RZ 1 containing a GFP (Green Fluorescent Protein) reporter gene and a ribozyme gene incising penaeid white spot baculovirus (WSBV) was first introduced into the fertilized egg of Chinese shrimp by gene gun. The treated and control samples of different development stages were observed with fluorescent microscope. The transient expression of GFP gene was high in nauplius and zoea larvae. Results from RT-PCR and PCR for adults showed that the foreign genes had been transferred into the shrimps and had expressed the corresponding proteins. Experiments on exogenous DNA (pGTR) transferring to Chinese shrimp convinced the availability for delivering foreign DNA by electroporator and showed that the hatchery rate of zygotes differed greatly under different treatments of voltage. Hatchery rate was inverse proportion to pulse length. The number of larvae from treated groups that had green fluorescence is very small. Under high voltage and short pulse length, such as 80V and O.5ms, only few individuals had green fluorescence, with very low hatchery rate. This work has established a transgenic method for penaeid shrimps, which will set base for the application of genetic engineering breeding into industry.