Random Amplified Polymorphic DNA Analysis Of Genomic DNA In Three Sheep Breeds And A Crossbred Population In Inner Mongolia Region

Random amplified polymorphic DNA (RAPD) analysis was used with 153 arbitrary 10-mer oligonucleotide primers to assess genetic variability of 28 individuals, which were from Wuzhumuqin sheep, German Mutton Merino, fine wool sheep of Inner Mongolia and a crossbred population . 38 out of the 153 primers can get clear RAPD fragments, of which 17 primers have polymorphic fragments. The 17 primers amplified 122 reproducible DNA fragments altogether through the polymerase chain reaction (PCR). The number of fragments of each primer is between 4-.--- 12.The molecular size of amplification products is between 500?2000bp.The results showed as follows: the genetic diversity coefficients within population were 0.3008, 0.1431, 0.2469 and 0.3074.The average coefficients of genetic diversity within population was 0.3822.The percentage of genetic diversity within population was 0.6535,among populations was 0.3465, so the genetic diversity was mainly existed within population. The genetic distance between all populations was between 0.0755?.1877.The affinity between crossbred and German Mutton Merino was distant while Wuzhumuqin sheep was close to fine wool sheep of Inner Mongolia . The result of molecular cluster analysis was accord with their affinity.