Studies On Cloning And Sequence Analysis Of The Porcine Myostatin CDNA

In order to study further natural functions of the porcine myostatin gene of which sequences were obtained from GenBank database and to establish theory basis of molecular breeding of porcine, a pair of upper, lower specific RT-PCR primers which containing 5 EcoR I and 3?BamH I restriction enzyme sites was designed. The full-length myostatin cDNA with 11 SObp of Sus scrofa breeding Habai was cloned by RT-PCR, sequenced and predicted its sequences of amino acid. We also compared the nucleotide and amino acid sequences homology of several Sus scrofa breedings with Blastn/Blastp.The results showed: Compared with four Sus scrofa breedings which are Yorkshire, Meishan~ Hampshire, Duroe, iespec Iveiy, Habai exists four point mutations. One was a T to (3 transversiou in 63 nt.of the myostatin coding region, not resulting amino acid change; the second was a T to C transition in 22nd resulting a substitution of Ala for Val (anino acid 41); the third was a (3 to A transversion in nt., resulting a substitution of Lys for Arg (anino acid 49) and the last was a C to T transition in int., resulting a substitution of lie for Thr (anino acid 56), respectively.The nucleotide sequences of Habai is 99% identical to the others in this region. On the other hand, there was no mutation in nt., but mutation in 634 nt. and the other three mutations were same, follwing the same amino acid changes. Finally, our results demonstrate that these amino acid changes didn cause the double-muscled phenotype. Postgradute: Liu Heping Specialty: Animal genetics and breeding Advisor: Associate Professor Liu Di...