Study On Maturation And Fertilization Of Buffalo Oocytes In Vitro

The buffalo has been identified as a domestic animal of great economic importance for Asia and Mediterranean. Although births of calves from embryos produced in vitro and transferred to recipient buffaloes have been recorded, only a few reports are available on the technology. Inherent problems of low follicular oocyte population coupled with fragility of buffalo semen result in low cleavage rate and morulalblastocyst production. Buffalo follicular oocytes fertilized and developed in vitro. The procedures for in vitro maturation, fertilization and culture of embryos in buffaloes must be improved and optimized. Nine experiments were conducted in this study to determine factors affecting the in vitro maturation (IVM) and in vitro fertilization (IVF) of buffalo oocytes. In experiment 1,2, the study was designed to evaluate the effects of area and types on ovarian follicle population and oocytes quality in buffalo and cattle. Results indicated that Henna's buffalo had lower number population and poor quality compared to Anhui's buffalo(p<0.05),the oocyte of buffalo was lower number population and poor quality than cattle(p<0.0 1). In experiment 3,the aim of this study was to elucidate the possibility of applying bovine IVF procedure on buffalo IVF. Aspiration of 379 buffalo ovaries has provided an average of 4.75oocytes per ovary, which is about 1/2 obtained from bovine ovary. The maturation rate (60%) and cleavage rate (25%) of buffalo ,oocytes were significantly lower (p