| In this paper, effects of fennel extract on pine wood nematode (Bursaphelenchus xylophilus) and its carrying bacteria were studied. Ethanol extract of fennel(Foeniculum vulgare Mill) was extracted by ethyl acetate after ethanol was evapotated and solved in distilled water. Nematicidal activity analysis indicacated that nematicidal componds were presented in the aqueous phase. Proteins from fennel, however, showed no nematicidal activity. Ethanol extract of fennel could kill pine wood nematode (PWN) efficiently with LC50 of 16.496mg/mL,9.508mg/mL,8.802mg/mL,7.845mg/mL, and 7.557mg/mL within 24,36,48,60,72h, respectively.Besides, the ethanol extract of fennel also showed antibacterial activity to most bacteria PWN carries at the concentration of 37.333mg/mL, with an efficiency of 77.3%. Pseudomonas fluorescens GcM5-1A associated with PWN was completely inhibited at the same concentration. Finally, three bacterial strains carried by PWN sensitive to and one strain resistant to the fenel extract were isolated and identified by morphology, physiology, biochemistry and 16S rDNA sequence blast. Three strains sensitive to the extract were identified as Klebsiella sp., Stenotrophomonas maltophilia, Streptomyces sp., respectively. The strain resistant to fennel extract was identified as Pantoea agglomerans. This research will offer some clues to the study on prevention of pine wilt disease using natural products which is environment-friendly. |
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