The Study Of Line Constructive Laser Confocal Scanning Microscope System

The confocal microscope can attain higher resolution and signal-to-noise ratio than ordinary optical microscope due to its"confocal"technique, and can make 3D images for the samples. Laser confocal scanning microscopes are widely applied in the areas of biomedicine, material science, microelectronic manufacture and precise measurement.Most types of confocal microscopes are using point cofocal scanning, while this dissertation presents a new type of line constructive laser confocal scanning microscope system. The main topics including:1,Point confocal microscopy 3D coherent transfer function(CTF) and line constructive laser confocal microscopy transmission cross-coefficient(TCC) are discussed based on Fourier optics and optical transfer function theory. The elements that affect the resolution of the image are analyzed.2,The optical system of line constructive laser confocal scanning microscope is designed. The optical design software OSLO is used to design special infinite image distance half flat field objective as well as laser extender and collimating lens.3,A machinery working platform movement flat scanning system that applies optical grating feedback closed loop control and an objective moving axial scanning system that applies stepping motor control and precise gear driving are developed.4,The integer configuration of the ling construction beam confocal microscope system is designed. The effect of light constructive laser confocal image is researched in experiment. The result testified that the line constructive laser confocal imaging can diminish the effect of stray light obviously, and the image articulation and contrast are improved. The system also has the ability of optical sectioning.During the two year's research work, we mastered the theory of confocal microscopy, and designed an experimental line constructive laser confocal scanning microscope device. All these works formed a nicer basis for the next work of improving imaging resolution and the development of image processing software.