Analysis Of Fatty Alcohols,phytosterols And Solanesol In Tobacco

This paper seeked to explore a routine analytical method to simultaneously determine free and esterified fatty alcohols, phytosterols and solanesol in tobacco, and apply this methodology to give comprehensive information about the contents and forms distributions of fatty alcohols, phytosterols and solanesol in various tobacco samples. Through the investigation of a lot of literatures and experiments, a simple and convenient gas chromatographic method equipped with flame ionization detection has been achieved for simultaneously determining six free and esterified fatty alcohols, three phytosterols, and solanesol in tobacco; explored the content and distribution of the target compounds in different types, varieties, planting locations and stalk positions of tobacco leaves. Moreover, the relationships between fatty alcohols, phytosterols, solanesol and fourteen PAHs in mainstream smoke were studied. These studies provided reference information for industrial and agricultural production of tobacco and cigarette, as well as further studies.Three main aspects were included in the thesis:1. An effective method using GC-FID for simultaneous quantitative analysis of free and esterified fatty alcohols, phytosterols and solanesol in tobacco leaves was established. A series of optimization have been done to achieve the most effective extraction, separation and determination of these target compounds in tobacco, such as saponification conditions, extraction conditions, derivation conditions and chromatographic conditions. This analytic method introduced direct alkaline hydrolysis, omitted laborious preliminary purification steps of samples prior to GC, and the method was simple and convenient. The limits of detection (LOD) of all target compounds varied from 0.1–0.4μg mL-1, recoveries were in the range of 85–105%, the relative standard deviations (RSDs) were less than 4%. The optimized methodology was successfully applied to determine free and esterified fatty alcohols, phytosterols and solanesol contents of different type tobacco samples.2. The contents of fatty alcohols, phytosterols and solanesol in tobacco leaves of various types, varieties, planting locations, stalk positions and nations were analyzed, and investigated the relationships between them. Meanwhile, the effect of various factors, such as genotype and growing environment to the contents of fatty alcohols, phytosterols and solanesol were also studied. The results provide useful information for evaluating the relationship between chemical composition and tobacco quality, as well as the planting and manufacture of tobacco.3. The relationship between fatty alcohols, phytosterols and solanesol in tobacco leaves and polycyclic aromatic hydrocarbons (PAHs) in mainstream smoke were explored preliminary, and seek the linear relationships between them. The results indicated a good linear correlation between phytosterols and benzopyrene. These studies provided reference for tobacco quality and safety.