| Pomelo is fruit of Rutaceae produced in fujian, jiangxi, guangdong, guangxi etc south area of China. Pomelo has rich nutrition and high medicinal value, but also the medical profession accepted as the most therapeutic benefits of fruit. Pomelo Peel is the by-product of Pomelo processing, rich in moisture, cellulose, lignin, trace elements and a variety of physiologically active ingredients, the main component is the pomelo peel dietary fiber (PPDF). DF as the seventh largest nutrients, with its special properties has been given more importance. China's DF-owned biomass is very impressive, but the utilization rate is very low. Reasonable PPDF preparation, can be used as functional materials, has wide application and development prospects. This article aims to select the best extraction methods of PPDF, study the function and the food safety of PPDF and the development of health care products for the PPDF provide experimental basis.Pomelo Peel without the pulp was used as raw material to extract dietary fiber by anzyme in this paper. It's optimized which the main process parameters, including the hydrolysis conditions for de-starch, fat removal, de-protein, decomposition PPTDF respectively into PPSDF and PPIDF by cellulose, finally gained the best process parameters for preparation and decolorization of PPDF. DF can adsorp lipids to enhance the lipid discharge and bile acids to enhance the lipid metabolism of discharge, which can reduce body fat content. It's analyzed the performance of its lipid absorption, the improvement of WHC and SWC and cation exchanging capacity,and anti-oxidative activity. In addition, its lipid-lowering activity in vivo of PPDF is in-depth research. It's proved that it has good lipid-lowering capabilities with the improvements of physiological indicators in hyperlipidemia rats with PPDF.1. With bio-enzymatic preparation of PPDF, amylase optimum digests time was 20min, as lipase and protease enzyme of the best time was 25min. Then cellulase was used to preparate PPSDF and PPIDF. When pH 5.5, the optimal cellulase hydrolysis process was as follows:hydrolysis time of 3h, cellulase dosage of 0.35μl/g, hydrolysis temperature of 60℃. PPSDF with PPIDF ratio is about 0.16. In addition, the optimal conditions of the decolorization of PPDF with H2O2 was as follows: decolorization time of 40min, H2O2 mass fraction of 6%, pH 8.0.2. Experiments for Physical and chemical nature of PPDF are carried out. First, its adsorption capacity of lipid material was researched. PPDF had a certain capacity of cholesterol absorption, and under conditions of high pH adsorption capacity was higher than the low pH conditions. Of fat and bile acids it had a certain adsorption capacity which for saturated fat more than unsaturated fat. Second, its capacity of WHC, SWC, and the cation exchange were better than the original untreated naringin powder, each of which increased by 15.13%,45.60%,29.29%. Third, PPDF had the antioxidant activity.It had certain reduction capacity and were able to remove a certain degree of O2-·. When clearing·OH, the IC50 was 3.63 mg/mL,as when clearing the DPPH·the IC50 was 8.06mg/mL.3. The function of moderating hyperlipaemia and losing weight to the Wistar rats was evaluated. The results demonstrated that the high concentrantion of PPDF could reduce their effective body weight by 12.8% and the serum total cholesterol (TC) by 23.4% and serum total triglyceride (TG) by 13.8%, increased high-density lipoprotein (HDL-C) by 53.4%, thus effectively declined the low-density lipoprotein cholesterol (LDL-C) by 38.6% and 60.0% of arteriosclerosis indices. It could significantly promote the fecal fat by 50.0% and reduce the rate of 37.9% of the body fat.4. The food safety of PPDF were assessed. The acute toxicity test confirmed the LD50 for PPDF was more than 15g/kg belonging to non-toxic level products. Micronucleus test of marrow cells and chromosome aberration test were carried out to judge the genetic toxicity. The results showed no induction of genetic anomaly for PPDF. By 30days of feeding test, it indicated that PPDF wouldn't cause any pathological effects. The results showed that there was no obvious toxicity, side-effect and dose dependently. Therefore, the PPDF was safe food according to the toxicological assessment on food safety.Based on the above points, enzymatic extraction of PPDF had a good yield, and the decolorize progress of PPDF had practical application value. WHC, SWC, cation exchange capacity of PPDF were significantly increased. It Showed the unique adsorption properties of lipids. PPDF had good lipid-lowering functions and was nontoxic, non-pathogenic teratogenic toxicity, no dose-dependent belonging to safety food. The study will provide a theoretieal basis for the functional effect of PPDF.
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