Bioinformatics Study On Microbial In Low-Temperature Anaerobic Granular Sludge

Microorganisms are the key organisms in the wastewater biological treatment systems. Within the wastewater treatment systems, a variety of microorganisms was coexisted and interacted. So, studying the microbial communities within these systems is very important for technique improvement and efficiency enhancement. Traditional techniques which is based on culture and separation could not satisfy environmental more directly and more exactly nowaday.With Human Genome Project(HGP) being brought intoforce, bioinformatics appeared and became the core areas of natural science in 21 century. By means of the bioinformatics research platform, a wealth of microbial resources can be researched real-time online and genome of environmental microbiology can be shared. Using molecular approaches based on 16S rRNA or rDNA sequence comparison, researchers can study microbes without culture and separation. And the results were gained fast and accurately.This test obtained the form, physiological and biochemical characteristics of low-temperature anaerobic sludge microorganisms relied on the traditional metchod of isolation and culture. By measuring 16S rDNA sequences, six sequences were gained. Application of emerging bioinformatics tools, 16S rDNA sequences of 6 strains were being analysised.Using Bioedit software, base composition and the DNA GC contents of six 16S rDNA sequences were analysised. By open reading frame search, DNA sequences were translated into their corresponding amino acid sequences. Using ClustalX progranm, multiple sequence alignment of six 16S rDNA sequences were made. Based on the comparison, the honologous of sequence was analysised and phyligenetic tree was reconstrut using the MEGA program. Analysising the kinship between unknown strains and other strains, we can learn the information of biological evolution .