Titanium Oxide Film Surface Modified By Plasma Ion Implantation Using Ammonia Gases And Immobilized With Biomolecular

In this work,Ti-O films were synthesized by Unbalance Magnetron Sputtering on silicon substrates,and then treated by plasma immersion ion implantation(Pâ…¢) using ammonia gases in order to introduce amino group to Ti-O film's surface.The structure and the chemical composition of the surface were analysed by atomic force microscopy(AFM) and X-ray photoelectron spectroscopy(XPS).The density of amino group on the surface of treated Ti-O films can be rationed by Acid Orangeâ…¡.After that,laminin(Ln) and fibronectin (Fn) were immobilized to the surface based on the amido group.The morphology of endothelial cells was observed by optical microscopy.In the end,human umbilical vein endothelial cells(HUVEC) primary culture experiments were adopted in vitro to evaluate the compatibility of the endothelial cells on the surface of the untreated Ti-O films,Pâ…¢-treated Ti-O films and the Ti-O films immobilized by biomolecules.The morphology of endothelial cells was observed by optical microscopy.The contact angle of Pâ…¢-treated Ti-O films surface was lower than that of the untreated Ti-O films.It indicates that the wettability of Pâ…¢-treated Ti-O films is a little higher than the untreated Ti-O films.The Acid Orangeâ…¡and XPS results show that the functional group of amido has been introduced to the surface of Ti-O film by plasma immersion ion implantation.The results of SEM and AFM suggest that the roughness of the Pâ…¢-treated Ti-O films surface has been increased.The result of immunofluorescence indicates that laminin and fibronectin has been immobilized to the Pâ…¢-treated Ti-O film surface.Endothelial cell culture experiment suggests that,the amino groups are helpful for the adhesion and proliferation of HUVEC.The Laminin and Fibronectin can further enhance HUVEC's adhesion,proliferation,and growth. According to the endothelial cell culture experiment,compared to the Pâ…¢-treated Ti-O films immobilized with Laminin or Fibronectin only,the Pâ…¢treated Ti-O films immobilized with both laminin and fibronectin can promote the adhesion and growth of endothelial.All these results indicated that the ion implantation can introduce functional group onto the surface of Ti-O film to change its properties like wettability and roughness,so it can increase the cell's adhesion.Laminin and fibronectin can be immobilized onto the surface of Ti-O film by amino group.Laminin and fibronectin can promote the adhesion and growth of endothelial cells.This work provide a good way for surface endothelialization by immobilizing laminin and fibronectin onto the surface of Pâ…¢-treated Ti-O film.