A Study On Quality Control Of Shexiang Zhuanggu Plaster

The phytochemical and pharmacological researches of some component on Shexiang Zhuanggu Plaster were summarized in the thesis. The method for the simultaneous determination of camphor, menthol, synthetic borneol and methyl salicylate in Shexiang Zhuanggu Plaster was established by gas chromatography. The sample solution was prepared by distillation with essential oil collector. Naphthalene is the internal standard. The GC system consisted of capillary column, PEG-20M as the stationary phase, nitrogen as the carrier gas, FID as the detector. The programmed temperature-GC and internal standard method was employed to determine four kinds of components in Shexiang Zhuanggu Plaster. Camphor, menthol, synthetic borneol (borneol and isoborneol) and methyl salicylate in Shexiang Zhuanggu Plaster and naphthalene have been separated well under the same chromatographic condition. The average recovery of camphor, menthol, synthetic borneol and methyl salicylate were 96.92%(RSD=2.42%), 98.03%(RSD=1.81%), 99.02%(RSD= 1.47%) and 98.15%(RSD=1.59%),respectively. The method is simple, accurate and separable, and can be used to control the quality of the four kinds of components.The article studied the effects of extraction condition on the percentage of the extraction of four components with essential oil collector. The probable range of the number of extraction, extraction time and water amount were obtained through one factor experiments, and the orthogonal experiments were designed based on one factor experiments. The results showed that the extraction obtained under the condition of number 1, time 4 h, water amount 150 mL has the highest percentage of the extraction.For the purpose of the limited determination of aconitine in Shexiang Zhuanggu Plaster, High Performance Liquid Chromatography method was established. The sample solution was prepared by three kinds of methods. External reference method was employed to determine aconitine in Shexiang Zhuanggu Plaster. The HPLC system consisted of Hypersil ODS2 C18 column, VWD as the detector. Detected Wavelength is 233 nm, Methanol-0.05% triethylamine(70:30) as flowing phase. The linear range was 0.08~1.60μg, r=0.9988. The detective limit was 3 ng, The precision for aconitine was less than 1.29% (n=6), The recoveries of aconitine was 98.65% (RSD=0.62%) in shexiang zhuanggu plaster respectively. The method can be used as a limited quantitative analysis of aconitine .