Studies On The Biological Properties Of Lactobacillus Reuteri And Function Fermented Milk

Lactobacillus reuteri is a kind of probiotics which is related to human health.In this study,the biological properties of Lactobacillus reuteri was studied,and the fermented milk containing Lactobacillus reuteri was developed,which had good taste and function.The results showed as follow.Lactobacillus reuteri had good grow characteristics.After it was incubated in 12.5%of recombinded milk for 12h at 37℃,the acidity could reach 67~OT and the viable count could be 2.3×10~8 cfu/mL.Under the condition of pH 2.0 or 0.5%of bile salt,60%of Lactobacillus reuteri was still survived after inculated for 5h.In the adherence test of intestine cell CaCO-2 in vitro,the adherence rate was 12.9±2.7%.The single-layer agar diffusion confirmed that Lactobacillus reuteri could significantly restrain the activities of E.coli.and H.pylori.The results indicated that Lactobacillus reuteri had good biological properties,could resist the acid and bile salt condition in human digestive tract,could adhere effectively on on the intestine cell,and could restrain the activity of intestineal pathagenic germs.So it could be used in functional fermented milk.The fermented milk contained Lactobacillus reuteri was developed.the optimal condition was that the concentration of whole milk powder was 12%,the ratio of Lactobacillus reuteri,Lactobacillus bulgaricus,Streptococus thermophilus was 0.5:1.5:1.5,the inoculability was 4%,the amount of sucrose was 8%,the combined stabilizer was 0.40%,fermented temperature was 41±1℃Cand time was 5,5-Th,The final pH was 4.4-4.5.The fermented milk contained Lactobacillus reuteri effectively resolved the flavor problems in common proiotics milk.The characteristic flavor in the product contained 11mg/kg of acetaldehyde,4mg/kg of dibutyl ketone,7500mg/kg of lactic acid and 193.1 mg/kg of acetic acid,and the acceptance of taste was above 85.When the product was stored in 0-7℃,The addition of the acidity to increase slowly,In 28 days Lactobacillus reuteri viable count still could be keeped above 10~6cfu/mL,The content of LAB-EPS reached 19.26 mg/kg.It was indicated that the fermented milk contained Lactobacillus reuteri have three function in this experiment:enhances the immunity,regulate intestinal bacteria balance as well as anti-oxidized.It is one of fermentated milk not only good taste and flavor but also some health functions.The biology characteristics of Lactobacillus reuteri was researched in this study,and its good performance was confirmed,So it could be used in functional food.Compared to the similar products,The fermented milk contained Lactobacillus reuteri not only had good flavor and taste,but also had many kinds of health functions.It has the good market introduction prospect and the economic efficiency.