Preparation And Characterization Of Chitosan Microspheres Loaded With 5-Fluorouracil

In this study,5-Fluorouracil(5-Fu)-loaded chitosan microsphere were prepared in emulsion by using glutaradelhyde as cross-linking agent,Span-80 as emulsifer and naphthene as oil phase. Microscope images showed that the decentralization and average roundness were influenced by many factors. By changing the conditions of experiment, the 5-Fu encapsulated capacity could achieve 40%, which was approach to that reported in literatures. In this experiment, UV spectrophotometer was used to determine the 5-Fu encapsulated capacity and many factors were calculated: the encapsulated capacity decreased with the density of acetic acid and the dosage of cross-linking agent. Drug releasing property of 5-Fu-CS microspheres was studied in the phosphate buffer (pH=7.0) and citrate buffer (pH=3.0) solutions. The results showed that m (5-Fu):m (CS),amount of cross-linking agent and the pH value of media all affected the releasing of 5-Fu from microspheres. The drug releasing rate was founded decreased with m (5-Fu):m(CS) and the increase of amount of cross-linking agent. The drug releasing performance was better in the acid media than in the alkali media.