Electrochemiluminescence Resonance Energy Transfer (ECRET) Of Luminol And Quantum Dot System

In chapter one of this thesis,a new method for resonance energy transfer between electrochemiluminescent donors and quantum dots(QDs)as acceptors was developed,which is called electrochemiluminescence resonance energy transfer (ECRET).In the ECRET,the emission of the emitters generated at an electrode through an electrochemical reaction is absorbed by donors.At the same time, fluorescence is emitted by the donors.We used the traditional luminol/H₂O₂ ECL system to generate the emitters and quantum dots(QDs)with a emission of 655 nm to serve as the acceptors.In this work,the conditions for performing ECRET including concentrations of luminol and H₂O₂,the method for immobilization of QDs on the surface of the transparent indium-tin oxide(ITO)electrode were investigated and discussed.It was found that when 1×10^-12mol for the amount of QD,0.9 V for the electrode potential,1.5 mm for width of slit,1×10^-3moi/L for luminol concentration and 1×10^-2mol/L for H₂O₂ concentration were used,the ECRET with a high energy transfer efficiency could be obtained.The advantage offered by ECRET is that no excitation light source is required to excite the donor,which avoids problems in FRET such as light scattering,high background noise,photobleaching,autofluorescence and direct acceptor excitation.In chapter two,we developed another ECRET system:luminol/glucose/glucose oxidase(GOD)-QD system.In this system,GOD catalyzes glucose to produce glucose acid and H₂O₂ in the presence of oxygen.Then,ECRET can be obtained according to the process described above.Optimum experiment conditions including pH value,electrode potential,the concentrations of glucose and GOD for ECRET of the system were investigated and discussed.We also investigated the ECRET for GOD in solution and immobilized onto ITO surface.