Preparation And Adsorption Property Of High Performance Adsorption And Detection Polymer Materials For Creatinine

The copolymerization of 2-hydroxyethyl methacrylate (HEMA),N-vinylpyrrolidone(NVP) and N,N'--Methylene bisacrylamide (MBA) was performed via inverse suspensionpolymerization method,and micron-sized crosslinking tripolymer microspheres were obtained.The effects of various factors,such as disperser kind,stirrer rate,ratio of oil phase to waterphase and the used amount of crosslinking reagent,on the forming of the microsphere and thediameter of the microspheres were examined.The chemical structure of the microspheres wascharacterized with FTIR spectrium,and their morphology was eobserved with scaningelectron microscope.The experimental results show that the micron-sized crosslinkingtripolymer microspherers with excellent sphericity and with controllable diameter in a rangeof 100～300μm can successfully prepared by using inverse suspension polymerizationsystem.The crosslinking copolymer microsphere was designated as HEMA/ NVP.MicrosphereHEMA/NVP was modified chemically with 3,5-dinitrobenzoyl chloride (DNBC) andfunctional microsphere DNBZ-HEMA/NVP on which a number of 3,5-dinitrobenzoate(DNBZ) group were bond was obtained.Its chemical structure and composition werecharacterized with FTIR and chemical analytical method.The adsorption characterization andmechanism of DNBZ-HEMA/NVP for creatinine were mainly studied.Static adsorptionexperiments show that the functional microsphere DNBZ-HEMA/NVP has strong adsorptionproperty for creatinine,and its maximum adsorption capacity is 20.56mg/g.Both pH valueand salinity influence the adsorption capacity greatly.When pH value is lower or higher,theadsorption capacity is smaller,and when pH=8.5,the adsorption capacity has maximum.Thegreater the salinity of the medium is,the smaller the adsorption capacity.The study resultsdisplay that the adsorption of the functional microsphere DNBZ-HEMA/NVP for creatinine is attributed to chemical adsorption,furthermore,it is a chemical adsorption driven byelectrostatic interaction.Grafting polymerization of methyl acrylic acid (MAA) was carried out on the surface ofsilica gel particles and grafting particles PMAA/SiO₂ were obtained.The static adsorptionexperiments of PMAA/SiO₂ for creatinine in aqueous solution were performed.The effects ofpH value and salinity of the medium and temperature were examined.The experimentalresults show that the grafting particles PMAA/SiO₂ have strong adsorption ability forcreatinine in aqueous solution.The pH value and salinity of the medium influence theadsorption property of PMAA/SiO₂ greatly.The adsorption of PMAA/SiO₂ for creatinine is aphysical adsorption and results from the synergisms of hydrogen bond and electrostaticinteraction between them.Between the above interactions,the electrostatic interaction is amajor droving force for the adsorption of PMAA/SiO₂ against creatinine.The molecularimprinted polymethylacrylic acid on the surface of silica gel was prepared using creatinine asa template molecule and ethylene glycol diglycidyl ether (EGDE) as crosslinking agent viacoordination bond effects,and composite molecular imprinted material MIP-PMAA/SiO₂ wasobtained.Both static and dynamic methods (i.e.batch and column methods) were adopted tostudy the binding properties of MIP-PMAA/SiO₂ for creatinine,and the effects of mainimprinting conditions,pH value and the amount of crosslinking agent,on the bindingproperties of MIP-PMAA/SiO₂ were examined.The experiment results show that themolecular imprinted material MIP-PMAA/SiO₂ possesses strong recognizing ability forcreatinine,and it is easy to be regenerated and reused.