The Studies On Subacute Toxicology Of NB In Mice

This paper selected contaminant nitrobenzene (NB) as research object, studied its nervetoxicity and the damage on oxidize defend system on mice with acetylcholinesterase(AChE),catalase(CAT), acetylcholine(ACh), glutathion peroxidase(GSH-Px),erythrocuprein(SOD),malonaldehyde(MDA), Total antioxidant capacity(T-AOC), proteincarbonyl content,change ofpathology, and cytochromeP450 at the molecular level of toxicity. Moreover, this paper tried toprobe into the effect mechanisms of its toxicity and oxidation damage in liver,kdeny,brian,spleen,which provided the basis for the fully estimate of ecological toxicology on mammals. The resultsshowed:1. Certified that NB belong to midrange accumulation by cumulative test.2. Observation of ultrastructural and show minute points, verified that NB can damage the ratliver, brain, spleen, kidney and degrade physiological functions. As judge and analyze toxicosiseffect, variance of pathology organization has offered sufficient morphology proof, after usedifferent dosage of NB.3. NB can induce rat liver, brain, spleen, kidney and other tissue oxidative stress, degradeactivity of CAT, GSH-Px, SOD and T-AOC, content of protein carbonyl and MDA higher, damageantioxygen defence system and function of clean up free radicle, and make tissues oxidativedamage, impact their normal physiological functions.4. With time prolonging and dosage adding, the activity of acetylcholine esterase in mousebrain containing degrade, content of liver acetylcholine to step up, and to show time and dosagedependence apparentely. It demonstrate that NB make effect on neurotransmitter and acetylcholine,and have neurotoxicity.5. By the detection of mixed function oxidase activity in rat liver, prove that NB can induce b5,Aminopyrine-N-demethylase,ethoxyresorufin-O-deethylase(EROD),Coumarin7-hydroxylation, incytochromeP450 enzyme system; abaissement NADPH-cytochrome C reductase, contributionorganism normal physio-function.NB can make liver, brain, spleen and nephridial tissue pathology damage, to refrain theactivity of liver mixed function oxidase, induce oxidative stress, effect tissue enzymatic activity,destroy organism antioxygen defence system and function of clean up free radical, to cause proteinoxidation damage, to educe toxic action.