Preparation Of Collagen Polypeptide Calcium And Its Application Research On Osteoporosis And Bone Growing

Collagen is one of the important and polyfunctional protein from pig skin. The optium hydrolysis conditions of pig skin with single enzyme and compound enzyme, and the suitable calcium chelating condition with collagen hyhrolysate were studied in this paper. Furthermore,animial experiments were used for validating the collagen polypeptide calcium was a first-rate Ca additive ,which was better than Calcium carbonate and calcium gluconate.Firsrtly, neutral proteinase, alkaline proteinase, papain were used to hydrolyze fresh pig skin respectively. The effects of pH value ,temperature, enzyme content and substrate content on hydrolysis were studied by single factor test and orthogonal, design with degree of hydrolysis (DH) as only guide was adopted, the optimum condition of hydrolysis parameters was determined. On the base of the optimum condition single proteinase, any two proteinases were chosen from the neutral proteinase, alkaline proteinase and papain, through the analysis of experiment date ,the optimum compounding were determined. The results showed as follows: (1) The suitable hydrolysis parameters of neutral proteinase was pH value 6.5,temperature 45℃,enzyme content 0.5%,suhstrate content 15%. The suitable hydrolysis parameters of alkaline proteinase was pH value 11,temperature 55℃,enzyme content 0.5%,suhstrate content 35%. The suitable hydrolysis parameters of papain was pH value 5,temperature 60℃,enzyme content 0.9%,suhstrate content30%.The ability of hydrolysis was alkaline proteinase >papain>neutral proteinase. (2) The compounding have the bigger DH than their single enzyme, and the sequence of addition inflents DH .The compounding of papain+alkaline has the biggest DH. (3) The result of the studied on the molecular weight of collagen hydrolysis and collagen indicate that the molecular weight of collagen is about 300000 daltons and narrow distribution, the molecular weight of collagen hydrolysis is smaller than collagen ,it's molecular weight is several thousands to forty thousands, and has a wide distribution.Secondly, the products of optimum compounding and the suitable calcium were choosed as the material of collagen polypeptide calcium ,according to chelated rate as only guide ,the synthetic conditions of calcium chelate was studied with an empHasis on the chelating reaction.The result showed that the optimal calcium was CaCl₂,the optimal reaction condition was pH value 8,the molar ratio is 4: 1.Consequently, the chelate products were purified and analyzed by common chemical method .Extration solvent was also evaluated .The effect of volume ratio of organic solvent to filtrate was studied.The highest recovery rate was obtained at 9:1 of ethyl alcohl to distilled water.The product was testified by ultraviolet (UV) and infra red (IR) . Lastly, The effect of collagen polypeptide calcium on the prevention and cure of osteoporosis and the promotion of bone's growing were testified by animal experiments.The result were showed that the collagen polypeptide calcium was one of the new supplement calcium which not only calcium but collagen, it's effect is better than Calcium carbonate and calcium gluconate on the prevention and cure of osteoporosis and bone's growing.