Differential Proteomics Study Of Microcystis Aeruginosa In Diverse N/P Concentrations

Microcystis aeruginosa is the vital specie of toxic cyanobacterial which frequently form dense growths known as blooms in eutrophicated waters. For this case, study of their proteins expression changes in simulant eutrophicated stage is very interesting and helpful to explore the mechanism of bloom formation.In our research, techniques of proteomic and bio-information were used to study the protein different expressions in mutative nitrogen and phosphorus concentration. Also, analysis the mechanism of different proteins in bio-metabolism aims at studying the different protein expression in the exact bloom environment.Firstly, to explore the optimal extract methods of Microcystis aeruginosa 7820, three different extraction methods were compared by two-dimensional electrophoresis. They were sonication extraction, TCA-acetone precipitation and sonication combined with TCA-acetone extraction. The 2-DE profiles showed a clear result that sonication (with Triton X-100) together with TCA-acetone appear sufficient for the extraction of proteomes of Microcystis aeruginosa and will be central to our ongoing efforts to examine the different proteome profile of Microcystis aeruginosa.Then, the primary molecular anatomic profile of was established, concluding 2-DE map and PMF map. Of 110 protein spots from the 2-DE gel, 21 spots could be matched with the genes or/and proteins of cyanobacteria in NCBI database, 11 of these could be matched with the genes or/and proteins of Synechococcus sp. PCC 6803 or/and Microcystis aeruginosa, which proportion is about 1:11. The molecular anatomization will provide useful data for further studies.Third, the protein profile of Microcystis aeruginosa in different concentration of nitrogen (1.6mg/L, 16.3mg/L, 245.1mg/L, 490.2mg/L)was analyzed. Limitation of nitrogen (1.6mg/L) in growth affected the synthesization of protein in a large degree. Compared to the normal controlled condition (nitrogen 81.7mg/L), six protein spots were down-regulated while one protein spots were...