Studies And Applications Of Fluorescence Spectrum On Pharmaceutical Analysis And The Interaction Of Drugs And Biomolecule

The application of molecular spectrum (including fluorescence spectrum and ultraviolet -visible spectrum) for the study on pharmaceutical analysis and the interaction between medicine and biomacromolecule is established. The thesis contains four parts:Chapter 1 reviews the progress of the fluorimetry in pharmaceutical analysis in recent years; The present situation of molecular spectrum for the reaction of medicine and biomacromolecule such as protein, nucleic acid. 134 documents are quoted (partly referred to 2004).Chapter 2 studys the application of fluorescence enhancement on the pharmaceutical analysis. These pharmaceuticals are triamcinolone acetonide(TA), indomethacin(IM), testosterone propionate(TP).1. A simple and highly sensitive method for the fluorescent spectrometry of TA has been developed by the effect of H2SO4, β -cyclodextrin( β -CD), solvent and cetyltrimethylammonium bromide(CTMAB) applied to it. Two new analytical methods are found. One is the system of CTMAB with its linear range 0~4.6X 10-6mol/L and the detection limit 3.59.10"8mol/L. The other is β-CD and ethanol, with its linear range 0~2.3 X10"6mol/L and the detection limit 1.91xl0~8mol/L. The two systems have successfully applied to the determination of TA in injection, the results are satisfied.2. The fluorescence properties of IM are probed by the effect of pH, β-CD and the cooperation of βi-CD and CTMAB. It is found that the fluorescence intensity of IM is greatly enhanced in pH=10.8, CTMAB and β-CD buffer solution. A new fluorimetric method for analysis of IM is developed, which shows high sensitivity with a detection limit of 5.73.10-9mol/L and a wider linear range of 0~2.79X 10-6mol/L between the fluorescence intensity and concentration for IM in aqueous solution. It has successfully been applied to the determination of IM in tablet, the result is satisfied.3. A simple and highly sensitive fluorimetric method for the determination of TP has also been developed by the effect of H2SO4, solvent and pH etc. applied to it. The best experimental condition is established. The linear of the method is 0~5.80.10"6mol/L and the detection limit is 2.01xl0-8mol/L, which has successfully been applied to the determination of TP in injection and the result is satisfied.Chapter 3 studys the application of fluorescence spectrum and ultraviolet-visible(UV-vis) spectrum on the study of interaction between pharmaceuticals(including indomethacin, mefenamic acid, doxycycline hyclate) and bovine serum albumin(BSA). With the quenching fluorescence spectra and nonradiative energy transfer theory, we study the quenchingmechanism and obtain the binding constant and the distance of reaction, et al. and discuss the interaction types between the pharmaceuticals and BSA.1. The binding characteristics of indomethacin(IM) and BSA have been studied by fluorescence and UV-vis absorption spectrum in aqueous solution. The results show that IM has a powerful capability to quench the BSA fluorescence via nonradiative energy transfer mechanism. The fluorescence quenching data are analyzed to obtain the binding constant and the reaction distance, et al. according to Stern-Volmer equation. From the synchronous spectrum IM holds some effect on the conformation of BSA to some extent.2. The binding characteristics of mefenamic acid(MEA) and BSA have been studied by fluorescence and UV-vis absorption spectrum in aqueous solution. The results show that MEA has a powerful capability to quench the BSA fluorescence via nonradiative energy transfer mechanism. The fluorescence quenching data are analyzed to obtain the binding constant, and the thermodynamic parameters et al. according to Scatchard equation and double-reciprocal equation.3. The binding characteristics of doxycycline hyclate(DH) and BSA have been studied by fluorescence and UV-vis absorption spectrum in aqueous solution. The results show that DH has a powerful capability to quench the BSA fluorescence via nonradiative energy transfer mechanism. The fluorescence quenching...