Study On The Isolation And Purification Of Ginsenoside Rb₃

ABSTRACT This paper dealt with the isolation and purification of the pharmaceuticalcomponent-ginsenoside Rb3 from the traditional Chinese medicine-Panax notoginseng,hoping to get the product of which the content can meet the need of the different newdrug and settling the material foundation for the new drug development. The notoginseng folium saponins were used as raw material and directly separatedby reversed phase preparative liquid chromatography. The effects of sample volume,construction and flow rate of the mobile phase on the separation process wereinvested. The suitable conditions were determined respectively. The content and thereceive efficiency of ginsenoside Rb3 in the crude product were about 65%~70% and20% respectively. In order to get the product (content>90%), the crude product was used as rawmaterial and separated again, through which the content of Rb3 in the product reachedabout 92.06%. To raise the yield, a semi-preparative chromatography was used whichis constituted of the following: the mix solution of methl cyanide and water as themobile phase, the C18 of 10μm particle diameter as packing φ40×300mm column.The primary preparative conditions were determined respectively. The content of theproduct Rb3 is about 86.2%, and which of Rc is above 95%. The general multi-component rate model of preparative chromatography wasestablished. The result of simulation showed good agreement with experimental datain regularity. The general multi-component rat model is very useful for guiding thepreparative process. The process for isolation and purification of ginsenoside Rb3 described here issimple and effective, and it will be very valuable to pharmacological research andnew drug development.