Study On The Methods Of Separation Of Magnoliae Officinalis Compositions Eliminating Active Oxygen Species And The Determination Of Elimination Activity Of Chinese Traditional Medicine To The Active Oxygen Species

Primary products storage and process engineering TIAN Yi-ling Abstract: The methods of eliminating three kinds of oxygen radicals (hydroxyl radical, superoxied anion and hydrogen peroxide) which can accelerate senile process were developed. They provide a simple path to choose the substances eliminating the oxygen radicals. It was also studied factors how to influence separation in nonaqueous capillary electrophoresis which is very lively in capillary electrophoresis. And a new method of separating magnolol and honokiol in magnolia bark was developed on the base.Benzoic acid with weak fluorescence may reacted on 'OH, and products with intense fluorescence are made. Extractives of Chinese traditional medicine may eliminate ?OH in solution, and make amounts of the products to reduce. Then, increase level of fluorescence of products in solution will be lowered. Based on this principle, a new method is developed to determine the IC50 of Chinese traditional medicine to ?OH. It is shown that productivity of ?OH tends to saturation when HiO: is shone more than 20 minutes by 280nm UV ray; ?OH may react on benzole acid completely when molar ratio of H2O2 to benzoic acid is 30:1; linear response range of products fluorescence is 2.2~80 mmol/L with concentration of H2O2. IC50 to eliminate OH with magnoliae and eucommia are 1.025mg/L and 515.3mg/L respectively. There are no remarkable difference between these results and that of spectrophotometry.Xanthine oxidase(XO) may catalyze the reaction of xanthine gaving uric acid and superoxide anion Cy") ?O2~can oxidize NET to form formazan. Extractives of Chinese traditional medicine may eliminate O2V in solution, and decrease the production velocity-51 -of formazan. A simple and sensitive kinetic spectrophotometric method to determination the ICso of Chinese traditional medicine to O2* is reported based on this principle. The effects of reaction time on absorbance and reaction velocity were studied. It is shown that sensitivity is higher from 1 min tol min. IC50 of vitamin C to O2vwas determined and compared with literature. The results are identical. ICso of Cortex Magnolias officinalis and Cortex Eucommia to CV are determined.Aniline may be oxidized to be nitrobenzene and its fluorescence quenched by h^O?. Based on this principle, a new method is developed to determine the 1C50 of Chinese traditional medicine to t^Cb. It is shown that fluorescence of aniline is close to maximum when pH of 50mmol/L phosphate buffer solution is 7.6, and this pH is consist with that of human blood. Scatter interference is minimum and fluorescence is maximum when concentration of ethanol is 50%(v/v). Linear relation is between quenching level of aniline fluorescence and concentration of H:O2 when aniline is HOmmol/L and H:C>2 is below Immol/L. The 1C50 of Cortex Magnolias officinalis and Cortex Eucommia to HUC^ are determined in the condition mentioned above. The results are identical with that of luminol chemiluminescence.A method was developed to separate magnolol and honokiol in Chinese tradition medicine Magnolia officinalis by nonaqueous capillary electrophoresis, which are principal elimination components to active oxygen species. It was studied how to choose nonaqueouse solvents, additive, and pH* and acid radical anion how to influence separation. The ratio of Acetonitrile to formamide to methanol is 12: 10: 3 (V: V: V), background electrolyte is 60 mmoI/L tris, and the concentration of water is 5%. The pH* of nonaqueouse buffer was adjusted by acetic acid. Magnolol and honokiol were separated under these conditions. The calibration curves were ?7685.56+11388265.36X and ?23744.94+17697231.73X, and correlation coefficients were 0.9996 and 0.9998 for-52-magnolol and honokiol respectively.