The Research On The Anti-tumor Effect Of Fusion Protein PTD4-VP3 In Cervical Cancer

Chicken anemia virus is a distinct type of single strand DNA virus. VP3 (apoptin) has shown to induce apoptosis in various tumor cells, but not in normal human cells and murine diploid cells. Apoptin-induced apoptosis does not dependent on functional P53, and cannot be inhibited by overexpression of Bcl-2, but caspase-3 activation is necessary for apoptin-induced rapid apoptosis. So VP3 is a very promising anti-tumor biological agents.Cell-Penetrating Peptide is a short peptide that can take along biological macromolecules into cells. Many research have showed that cell-penetrating peptide can successfully mediate the introduction of biological macromolecules, such as polypeptide , GFP, RNaseA, DNA, polar molecule and lipid body. So it has the potential to deliver both existing and novel anticancer therapeutics. This research combined the two aspect to discuss the anti-tumor effect of the fusion protein.This research is based on the constructing of the prokaryotic expression vector of PTD4-VP3. The fusion protein is reduced by IPTG, and the purification of the fusion protein. The PTD4-VP3 induced apoptosis of tumor cells in vitro was tested by FACS. We construct the nude mice models of cervical cancer by Hela cells. The anti-tumor effect of PTD4-VP3 fusion protein in vivo was tested by TUNEL assay and comparison of the tumor size after treatment. The result showed that PTD4 can take along fusion protein VP3 into cells, and induce apoptosis. The rate of apoptosis is the highest at 48h. The fusion protein can inhibit tumor growth by inducing apoptosis. This research will be the base for the study of the anti-tumor therapy.