Isolation Of Microsatellite Loci From Pratt's Leaf-nosed Bat (Hipposideros Pratti) And Cross-species Amplification

Microsatellite DNA is a widely used molecular marker.The success rate of isolating microsatellite loci from a specified species is getting higher and higher as a result of the advance of related technology.In this study we isolated 16 microsatellite loci from Hipposideros pratti by using a genomic enriched library for AC dinucleotide repeats,and tested the polymorphism on a population from Sichuan Province,China.The screening results showed that four loci were monomorphic and three loci were hardly genotyped explicitly.The remaining nine microsatellite loci displayed high levels of polymorphism.A total of 91 alleles was tested with 10.1 per locus(ranging from 4 to 14).The observed(H_O) and expected heterozygosities(H_E) ranged from 0.375 to 0.966 and 0.513 to 0.886,respectively.Three microsatellite loci (PB12,P9 and P02) revealed significant deviations from Hardy-Weinberg equilibrium after Bonferroni correction for multiple tests(adjustedα=0.0056,k=9).An analysis using Micro-checker v2.2.3 suggested that these deviations were due to the presence of null alleles.No significant linkage disequilibrium was detected between any pairs of loci.Owing to the high applicability of microsatellite markers in closely related species,we tested on Hipposideros pratti using eight microsatellite markers of Hipposideros armiger.Of them,five loci displayed stability of amplification and high levels of polymorphism.In conclusion,14 microsatellite loci will be used to study population genetic structure and conservation strategies of H.pratti.In addition,9 microsatellite markers of H.pratti were tested for potential cross-amplification in related congeneric and confamilial species,8 loci indicated high applicability,providing more molecular markers on studying population genetics in closely related species.