Fuctions Of Protein Phosphatases PP2C Genes On Salt Tolerance And Carbon Utilization In Saccharomyces Cerevisiae

A previous study in our laboratory has found that deletion of either PTC1 or PTC6 causes yeast cells sensitive to rapamycin[1]. In order to find if there is a genetic interaction between PTC1 and PTC6, we examined the phenotypes of PTC1 and PTC6 single or double gene deletion mutants in the presence of nonfermentable carbons, rapamycin, LiCl, NaCl and KCl. We found that deletion of PTC1 suppressed the phenotypes of the ptc6 mutant, and that overexpression of PTC1 in the ptc6 mutant increased the tolerance level to rapamycin. In addition, we did not observe any genetic interaction between PTC1, PTC5 and PTC7 in the presence of LiCl, NaCl and KCl,but there was a interaction between PTC5 and PTC7 in rapamycin sensitivity.