Study On Phylogenetic Relationships Of Partial Lonicera L. Based On NrDNA ITS Regions

In carries on the plant member systematic research process, the choice of suitable evolution speed of the gene or the DNA fragment is extremely important regarding the system growth relations inference. Because plant ribose the gap area (nrDNA ITS) in the cell nucleus evolves the speed very quick, may provide the richer information bit spot.At present it has been widely applied to the research angiosperm, inter-species and so on.The domestic and foreign scholars have more differences in the Lonicera phylogenetic, therefore, there has the necessary to carry on the discussion.This research take the nrDNA ITS sequence as the object, to mensurate ITS area complete sequences of Lonicera plant in Xinjiang's 8 kind and the introduction 7 kind, 5 Lonicera plant ITS sequences data obtained in GenBank which using system growth analysis software MEGA3.1 to carry on the analysis, and has carried on 37 shapescharacters quantity classification analysis to above 15 kind of Lonicera plant and 1 out-group. Obtained result as follows:1.The nrDNA ITS sequences of the 15 Lonicera plant and one out-group were obtained by primer designing, PCR, cloning and sequencing, which subsequently were used to analyze the phylogenetic relationships in the genus by homologous blast and clade analysis.2.In the data set of 15 Lonicera plant, the percentage of phylogenetically informative sites is 11.7% in ITS regions (including two spacers, without 5.8S spacers).In the data set including the ITS sequences downloaded from GenBank, the percentage of phylogenetically informative sites is 11.0%. According to the above statistics, there ate relatively abundant informative sites in the ITS sequences of genus Lonicera. The phylogenetix analyses based on the ITS sequence are in consensus with the morphological classification of the group within the genus to a degree, which proved that the ITS regions are valuable to resolve the phylogenetic relationships in Lonicera.The quantity classification result basic is also consistent with the ITS area analysis result, from this has also proven the ITS area in the Lonicera plant classification the feasibility.3.Obtains from the ITS sequence analysis respectively gathers charts and quantity classified collection class chart, L.caerulea var. altaic, L. caerulea var.caerulea and L. caerulea var. edulis gather in one branch, Bootstrap reach as high as above 90%, the ITS data, the quantity classified data showed that L. caerulea var. edulis, L. caerulea var. altaic and L. caerulea var. caerulea to be the Sect.Caeruleae, tallies with the traditional classification, therefore will not support L. caerulea var. edulis to stand newly is.4.In to 20 kind of Loniceras plants' analysis, based on the ITS sequence data genealogical tree, supports in the traditional classification to the Lonicera Sect.Coeloxylosteum, Sect.Nintooa and Subsect.caerulea the division, obtains high Bootstrap (bootstrap> 90%), other three groups'divisions must increase the research texons to conduct the further research. 5.In the genealogical tree which constructs to 15 kind of Lonicera plants ITS sequence, in the NJ genealogical tree which including GenBank in as well as which publishes ITS the area sequence 20 kind of Lonicera department ITS sequences construct (to have a tree), in the Lonicera an each group of group has not displayed first branches out from the base, therefore has not formed the genealogical tree the base branch in, endures to be each group to form the sisters group relations, displays the parallel evolution the tendency.