Studies On Establishment Of In Vitro Plant Regeneration System From Bulblets Of Lilium Lancifolium

The present study was carried out to establish a plant regeneration system of Lilium lancifolium and bulblets were selected as the explants. One part of budding spots formed was transferred into rooting media to induce plantlets. The other part was transferred into media to induce calli. Results of the study would provide fundamental supports to induction, domestication and culture of seedlings at a factorial scale, and to studies on medicinal ingredients of Lilium lancifolium.Results show that compared with other auxin-like growth regulators NAA (α-naphthaleneacetic acid) was the most sensitive one for inducing shoots from the bulblets of Lilium lancifolium. The best formula for primary culture of the buldlet scales was MS+NAA 1.0 mg l^-1 +BA (N⁶-benzyladenine) 0.1 mg l^-1, that for regenerating culture was MS+NAA 1.5 mg l^-1+BA 0.5 mg l^-1, and that for rooting culture was MS+IBA (indole-3-butyric acid) 1.0 mg l^-1. Upon transplantation of the in vitro plant seedlings, the survival rate in vermiculites was 85%, much higher than that transplanted in sandy soils.It is found that induction rate of callus was relative higher when the concentration of BA was comparatively high. Induction rate was the highest when formula of the culture medium was MS+BA 1.5 mg l^-1+NAA 0.5 mg l^-1. At the same time, the results indicated that dark condition was favorable to the growth of callus and change of contents of macroelements in the medium had no obvious influence on its growth. Rates of bud formation from well-grown callus was the highest in medium with a formula of MS + 2,4-D 1.5 mg l^-1+BA 0.1 mg l^-1 . Yields of growth of suspension culture cells were the highest with no serious brown when basic medium was supplemented with sucrose of 20 g l^-1, inositol of 150 mg l^-1 and half amount of macroelements compared with those in the original medium.