Taxonomic Studies On Thermophilic Fungi And Purification And Properties Endocellulase And β-glucosidase From T.aurantiacus Var.levisporus

Thermophilic fungi with unusual ability can survive under hightemperature condition, producing special metabolization such asthermozymes, and it is considered to be essential microorganism source forstudying organic evolution and thermozymes.The first part of this study ismainly about the taxonomic research of thermophilic fungi in China.Wecollected and checked a sum of over 600 specimens from 2002 to 2004,from which about 50 isolates were received. We discovered quite a fewnew and noteworthy taxa and achieved many valuable results based on thecareful identificatin. 19 taxa at species level are described and illustrated.Among them, there are 1 new species, 9 Chinese newly recored species, 8reported species from China including 4 thermotolerant fungi.Besides the new species, new record species discovered, we counted thehabits and distributions of the species respectively, discussed andsummarized the similar species. As a result, some valuable taxonomicmethods, criteria and our viewpoints were concluded. The morphology ofthe sporus is the main identical base at species level, which include thecharacters of the shape, size, colour, septate and ornament of the wall etc,and some sporus with distinct variable are also useful at genus level.Secondly, the characters of the conidiophore, the extending mode of theconidiogenus cells and conidiogenous pattern are key to distinguish thesefungi at genus level. In additin, the culture features and sporulation patternare essential and helpful complement for identification work..Thermoascus aurantiacus var. levisporus can be well thrive attemperature between 45 and 50℃, cellulases from it have reported to keephigher activity and thermostibility The second part of the study mainlydiscussed the purification and characterization of endocllulase.and β-glucosidase from Thermoascus aurantiacus var.levisporus.The endocellulase and β-glucosidase from culture supernatant waspurifided to homogeneity by using ammonium sulfate fraction, DEAE-Sepharose chromatography, Pheny1-Sepharose Fast Flow chromatographyand SephacrylS-100 chromatography. The molecular mass of the...