The Neutrophils Antigen Gene Allele Frequency Of Uygur Han, Kazak People In Urumqi Region

Objective: To study the neurophils antigen gene allele frequency of Uygur, Han, Kazak nationality people in Urumqi region for gene diagnosis of some transfusion diseases.Methods: A optimum multiprimered PCR-SSP (plymerase chain reaction with sequence-specific primers) method was established to screen the netrophils antigen (NA) genotype of DNA samples isolated from 120 Uygur, 118 Han, 102 Kazak nationality people blood donors in Urumqi region.Results: The allele frequency of NA1 of Uygur, Han, Kazak was 0.383,0.555,0.510 and NA2 was 0.617 ,0.445,0.490 in this sample volume. The statistical figures show that Uygur and Han has polymorphism of NA gene(P<0.05). Uygur and Kazak, Kazak and Han hasn't polymorphism of NA gene(P>0.05).Conclusion: The multiprimered PCR-SSP method is confirmed to be reliable and repeatable in our experiments for NA allele typing.