Association Between Coagulation Factors Related Gene Polymorphism And Venous Thromboembolism Risk In Kazak Pregnant Women In Xinjiang

Objective:Venous thromboembolism(VTE)is a common and frequently-occurring disease in xinjiang.From 2011 to 2014,goxiaohu et al.found that kazak population in xinjiang had genetic and environmental factors of Hereditary thrombosis,Which reveals the F Ⅴ(coagulation factor Ⅴ),F Ⅱ(coagulation factorⅡ)gene polymorphism may be associated with Hereditary thrombosis;It was also confirmed that smoking,age,and fresh vegetable intake were possible environmental factors for hereditary thrombosis.Preliminary studies have shown that the activity of protein C(PC)and protein S(PS)in kazak pregnant women is lower and has a higher incidence of disease.What is the incidence of pregnant women of kazak nationality in xinjiang suffer from venous thromboembolism?Is it related to environmental factors and/or genetic factors?Which genes are involved?IN this study,by detecting antithrombin-Ⅲ(AT-Ⅲ),PC,PS,activated protein C resistance(APCR),and identifying candidate genes,to study the relationship between the lack of anticoagulant protein,candidate genes and the pregnant women of kazak nationality in xinjiang.Methods:Part One: A cross section study was carried on the xinjiang kazak permanent,combined with epidemiological investigation questionnaire,physical examination and ultrasonic imaging test results,analysis the crowd of environmental factors,personal habits,information obtained according to the data types of computing the prevalence of venous thromboembolism,studies the influence factors of the disease,the results into the multi-factor logistic regression model for multiple factors analysis,comprehensive analysis of environmental risk factors associated with the disease.Part Two: In this study,the prevalence of venous thromboembolism + hereditary thrombosis in pregnant and postpartum women in the kazakh nationality was obtained through the measurement and analysis of 976 cases of maternal anticoagulation indicators,and the proportion of AT-Ⅲ defect,PC defect,PS defect,APCR and high Hcy was determined.Part Three: The selected genetic hereditary thrombosis+venous thromboembolism disease patients as the case group,other cases as control group,the case-control study method,select the tag SNP genotyping by using Sequenom Massarray detection technology,a clear clotting factors related gene polymorphism and kazak maternal hereditary thrombosis+venous thromboembolism.Results:(1)The prevalence of hereditary thrombosis+venous thromboembolism in the kazak pregnant women in xinjiang was 33.8%,among them the AT-Ⅲ deficiency,deficiency of PC,PS deficiency and APCR and the prevalence of Hcy,respectively,17.5%,16.7%,22.0%,23.7% and 26.8%.(2)Age and high fresh meat intake are the possible influencing factors of hereditary thrombosis +VTE in pregnant women of kazak nationality.(3)Polymorphisms of the prothrombin genes rs3136447 and rs5896 may be related to the hereditary thrombosis of the kazakh people in xinjiang.Conclusion:This study clarified the prevalence of venous thromboembolism in the special population of pregnant and parturient women of kazak nationality in xinjiang,and revealed the incidence of AT-Ⅲ deficiency,PC deficiency,PS deficiency and APCR.Once again,It has verified and confirmed that obesity has no significant impact on the development of venous thromboembolism in the pregnant and postpartum population of kazak nationality.The sudden change of lifestyle,higher homocysteine and lower intake of fresh vegetables were confirmed again as possible influencing factors for the development of venous thromboembolism(vte)in the kazakh maternal population.The clinical and genetic information was collected for comparison with other regions and ethnic groups,provides a basis for long-term follow-up study and/or prevention and treatment of venous thromboembolism.