Relationship Between Airway Microbiota And SUVmax,Prognosis Of Patients With Advanced Non-small Cell Lung Cancer

Background:.Lung cancer is a malignant tumor with the highest mortality.Previous studies have confirmed that airway microbiota is correlated with pathological types,clinical stages,and mutated gene types of patients with non-small cell lung cancer(NSCLC).However,few studies have addressed the relationship between airway microbiome and prognosis of NSCLC patients.Objective:First,we aim to investigated whether the primary metabolic response were associated with pulmonary microbiome in patients with advanced NSCLC.Second,we describe the pulmonary microbiome in patients of advanced NSCLC with different prognosis outcomes and confirm the abundance of specific taxa affecting the prognosis of NSCLC patients.Methods:We collected sputum samples of 97 patients with NSCLC.16S rRNA technology was used to sequence the v3-v4 region of pulmonary microbiome.NSCLC patients were divided into high SUVmax(SUV_LH group)and low SUVmax(SUV_LL group)according to the median of maximum standard intake value(SUVmax)of PET.According to the respond evaluation criteria in solid tumor,patients with advanced NSCLC were evaluated by imaging lasting the one year.Responder status(R group)was defined by patients with Complete Respond(CR)+Partial Respond(PR)+stable disease(SD)>1 year.Patients with Progress Disease(PD)within 1 year,were included in non-response group(NR group).Until January 2021,patients with advanced NSCLC were divided into 1-year death group(OS_L1 group)and 1-year non-death group(OS_H1 group).Bioinformatics was used to describe the distribution characteristics of pulmonary microbiota.Results:1.Alpha diversity and beta diversity between SUV_LL group and SUV_LH group was similar.There was a correlation between specific taxa of sputum and SUVmax with advanced NSCLC.The relative abundance of Pseudomonas was higher in low SUVmax group.2.Alpha diversity and beta diversity between NR group and R group was similar.The relative abundance of specific taxa of sputum was related to the efficacy of targeted therapy with advanced NSCLC patients.The relative abundance of durum in NR group were significantly increased.3.Alpha diversity and beta diversity between OS_L1 group and OS_H1 group was similar.The relative abundance of specific taxa of sputum was associated with survival time with advanced NSCLC patients.The relative abundance of Alphaproteobacteria was significantly increased in OS_L1 group.4.Alpha diversity and beta diversity between MOS_L1 group and MOS_H1 group was similar.The relative abundance of specific taxa of sputum was related to survival time with NSCLC patients with distant metastasis.The relative abundance of Alphaproteobacteria was significantly increased in MOS_L1 group.Conclusions:The relative abundance of specific taxa of airway microbiome was correlated with SUVmax,treatment respons and prognosis of advanced NSCLC patients.It suggested that the change of the relative abundance of bacteria in sputum may be a potential prognostic marker for advanced non-small cell lung cancer.