| Objective:To observe the effect of Shenqi Yixin Fang on the cardiac function of CHF rats,and to explore the mechanism of Shenqi Yixin Fang in improving myocardial fibrosis in rats with Galectin-3 and sST2.Methods:Healthy male wistar rats were randomly divided into six groups:normal group,model group,western medicine control group,Chinese medicine control group,high dosegroup,low dosegroup.The animal models of CHF were induced by intraperitoneal injection of adriamycin,and the success of the model was evaluated by echocardiography and BNP.After the model was established,the rats were treated with the corresponding drugs for 4 weeks.To observe the general condition and mortality of the rats in each group,the morphological changes of myocardium and the degree of fibrosis in rats;The cardiac function of each group was detected by echocardiography.The levels of serum BNP,Galectin-3 and MMP were detected by Elisa;The expressions of Galectin-3,MMP-9,TIMP-1,collal and col3α1 mRNA in myocardium were detected by RT-PCR.The protein expression of sST2 and IL-33 in myocardium of rats were detected by Western blot.Results:1.The levels of LVIDs,LVIDd,LVEF and LVFS in the model group were significantly different from those in the normal group(P<0.05).After 4 weeks of drug intervention,the levels of LVIDd,LVEF and LVFS in the treatment group were improved.There was significant difference between the high dose group,the western medicine control group,the Chinese medicine control group and the model group(P<0.05).2.The levels of serum BNP in the model group were significantly different between the normal group(P<0.01);After 4 weeks of drug intervention,the levels of serum BNP in the treatment group were lower than those in the control group(P<0.05).3.HE staining showed that the myocardial cells in the model group were partially edema,and some of the cardiomyocytes were small focal or lamellar necrosis.Some of the myocardial fibers were broken,and the myocardium was congested and infiltrated with inflammatory cells.After 4 weeks of drug intervention,the high dose group,western medicine control group and Chinese medicine control group compared with the model group had significant improvement in myocardial damage;Masson staining showed that the fibrosis of the myocardium was serious in the model group.The collagen fibers in the model group were significantly lower than those in the control group.4.The level of Galectin-3 and the expression of Galectin-3 mRNA in the model group were significantly higher than those in the normal group(P<0.01).After 4 weeks of drug intervention,the serum Galectin-3 level and myocardial tissue Galectin-3 mRNA expression levels were decreased in different degrees,compared with the model group were statistically significant(P<0.05).5.The level of MMP-2 and the expression of MMP-9 mRNA in the model group were significantly higher than those in the normal group(P<0.01),and the expression of TIMP-1 mRNA in the myocardium was significantly decreased(P<0.01).The expression of MMP-9 and MMP-9 mRNA in the serum of MMP-2 and myocardium were significantly decreased after 4 weeks of drug intervention(P<0.05).The expression of TIMP-1 mRNA was increased in different degrees,compared with the model group(P<0.05).6.The expression of collal and col3α1 mRNA in the myocardium of the model group was significantly higher than that of the normal group(P<0.01).The expression of collal and col3α1 mRNA in the myocardium of the treated group was significantly lower than that in the control group,the difference was statistically significant(P<0.05).7.The protein expression of sST2 and IL-33 in the model group was significantly higher than that in the normal group(P<0.01).After 4 weeks of drug intervention,the expression of sST2,IL-33 of the high dose group,western medicine control group and Chinese medicine control group were significantly lower than that in the model group(P<0.05).Conclusion:1.Shenqi Yixin Fang through reduce the CHF rats serum Galectin-3,MMP-2 levels and myocardial tissue Galectin-3,MMP-9,col1α1,col3α1 mRNA expression level effectively,at the same time increase the myocardial tissue TIMP-1 mRNA expression level,to improve the degree of CHF myocardial fibrosis in rats.2.Shenqi Yixin Fang through reduce the CHF rats myocardial tissue ST2 protein and IL-33 protein expression levels,may participate in and influence the ST2/IL-33 signaling pathways,to improve the degree of CHF myocardial fibrosis in rats.3.Shenqi Yixin Fang is an effective prescription for the treatment of CHF by improving the myocardium morphology and fibrosis in CHF rats,and improving the cardiac function of CHF rats. |
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