Soybean GmPAL Gene Family Enhance The Resistance Of Soybean Against Heterodera Glycines

Soybean cyst nematode disease（SCN）seriously affects the yield of soybean,and causes billions of dollars of losses every year.The most economical and effective control method in the world is to plant disease resistant varieties.Further,it is urgent to develop new resistant variety resources and resistance genes.Previous studies shown that resistance of soybean are can regulated by amino acid and hormone metabolism.Besides,soybean cyst nematode also affects aromatic amino acid metabolism by secreting branched acid mutase effectors.Those studies suggest that the metabolism of aromatic amino acids is closely related to the resistance of soybean to cyst nematode.Previous studies noticed that phenylalanine ammonia lyase（PAL）is related to resistance through transcriptome sequencing.However,the relationship between PAL and resistance is still unknown.To verifying,a pathosystem of fluorescent hairy root versus soybean cyst nematode（FHR-SCN）was constructed,which causes less time in experiments.Based on upon research progress of this dissertation are provided as follows.1.Overexpression of GmPAL gene enhanced the resistance of soybean against cyst nema-tode.The GmPAL gene were mined from transcriptomic data.Expression pattern assay showed that GmPAL protein mainly located in cytoplasm and membrane.The resistance of soybean against soybean cyst nematode was suppressed under L-AOPP treated.And expression of eight GmPAL genes caused the diapause nematodes in roots.The contents of isoflavones such as daidzein and genistein were significantly increased after the expression of GmPAL gene.Fed daidzein and genistein into soybean from petiole enhanced the resistance against soybean cyst nematode and inhibited the development of nematodes.2.Aromatic amino acids affect the resistance against soybean to cyst nematode.The content of phenylalanine increased in transgenic FHRs.Petiole feeding showed that phenylalanine could enhance the resistance.3.Overexpression of Gm HS1^pro-1 enhanced soybean resistance against soybean cyst nem-atode.Structural analysis showed that Gm HS1^pro-1 gene may encode tryptophan dioxygenase to catalyze tryptophan and auxin.Overexpression of Gm HS1^pro-1 gene enhanced the resistance against soybean cyst nematode.Moreover,Gm HS1^pro-1 gene reduced the content of tryptophan in transgenic soybean roots and increased the contents of JA and JA-Ile.These results showed that GmPAL gene and Gm HS1^pro-1 gene enhanced the resistance against soybean cyst nematode,which has application potential.Treatment of these isoflavo-noids also improved the resistance of soybean against soybean cyst nematode,indicating that isoflavones play a role in the process of soybean resistance,which provides a new idea for the control of soybean cyst nematode.In addition,the p NI9000 plasmids constructed in this disser-tation has independent intellectual property rights.The polycistronicm expression system of soybean hairy roots could be used with 2A self-cleaving peptide.The vector can also be used for the study of protein interaction.