| ObjectivesTo compare the hemodynamic changes of long-chain triglyceride emulsion and long-chain/medium-chain triglyceride emulsion when treating propafenone poisoning in rats,and explore related mechanisms.Methods1.Experiments were carried out in 8-10 week old male SD rats,30 rats were divided into long-chain triglyceride emulsion group（LL group）,long-chain/medium-chain triglyceride emulsion group（ML group）and normal saline group（NS group）,each group contain 10 rats.After anesthesia,tracheal intubation,carotid artery catheterization and jugular vein catheterization were performed in rats,mean arterial pressure and heart rate were recorded continuously by a Powerlab data acquisition system.2.After stabilization,propafenone was infused in the jugular vein,when the mean arterial pressure dropped to half of the base value,the time was recorded as 0 min,stop propafenone infusion propafenone immediately,the rats received lipid emulsion or normal saline 1.5ml/kg as a bolus followed by infusion for 60 min at0.25ml.kg-1.min-1.Draw 0.5ml blood sample from carotid artery at 5min,15min,25min,60min,plasma free propafenone concentration was determined by high performance liquid chromatography.At 60min arterial blood gas analysis was performed,and the apical tissue was immediately taken for concentration detection by high performance liquid chromatography after the rats were executed.3.In vitro,propafenone was added to rat plasma at a concentration of 3.5μg/ml,and long-chain or long-chain/medium-chain triglyceride emulsion was added to the plasma at a volume ratio of 1%,2%and 4%of the total volume,respectively.After centrifugation,the concentration of free propafenone in plasma was determined by high performance liquid chromatography.Result:1.The blood pressure and heart rate of group LL were significantly higher than those of group NS after detoxification treatment（P<0.05）.The heart rate of group ML was slower than that of group LL and group NS,and the difference was statistically significant（P<0.05）.The blood pressure of ML group increased rapidly within 5minutes after detoxification treatment,and decreased gradually after 10 minutes.The blood pressure of ML group was higher than that of LL group and NS group within 25minutes,and lower than that of LL group and NS group after 30 minutes（P<0.05）.2.At 60min the PH value and HCO3-in ML group were significantly lower than those in LL group and NS group,and the lactate value in ML group was apparently higher than those in LL group and NS group（P<0.05）3.The concentration of free propafenone in plasma of the three groups declined obviously with the prolongation of time.At 60min the concentration of propafenone in LL group was significantly lower than that in NS group（P<0.05）and that in ML group was lower than that in NS group（P>0.05）.The concentrations of propafenone in LL and ML groups were lower than those in NS group at 15min,25min and 60min（P<0.05）,and there was no significant difference between LL and ML groups（P>0.05）.4.The concentration of propafenone in myocardium of LL group was the lowest,while that of NS group was the highest,and there was obvious difference among the three groups（P<0.05）.5.In vitro,with the increase of lipid emulsion concentration,the concentration of free propafenone gradually decreased.The concentration of free propafenone in LL group was lower than that in ML group.There was no significant difference between the two groups when the concentration of lipid emulsion was 1%（P>0.05）.The difference between the two groups was notable when the concentration of lipid emulsion was 2%and 4%（P<0.05）.Conclusions:1.The long-chain/medium-chain triglyceride emulsion causes cardiac inhibition,which leads to the failure of detoxification in propafenone poisoning.2.The effect of long-chain triglyceride emulsion is better than that of long-chain/medium-chain triglyceride emulsion,which is suitable for detoxification of propafenone poisoning. |
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