| Objective: To study whether ginsenoside Rg1 can promote the recovery of neurological function after spinal cord injury in rats.Methods: A total of 80 female SD rats were selected,and a moderate spinal cord injury model was established by modified Allen method.This experiment was divided into four groups: blank control group,blank control group + ginsenoside Rg1 intervention group,spinal cord injury modeling group,spinal cord injury +ginsenoside Rg1 intervention group.The spinal cord sections were stained with HE at different time points after injury to observe the degree of inflammation of spinal cord tissue and record the BBB score of hind limb function at each time point.Results: HE staining of spinal cord slices showed that the degree of inflammation and the area of syringomyelia in the ginsenoside Rg1 intervention group were significantly smaller than those in the spinal cord injury group.The recovery of hindlimb motor function in the ginsenoside Rg1 intervention group was significantly better than that in the spinal cord injury group.Conclusion: Ginsenoside Rg1 can significantly promote the recovery of neurological function after spinal cord injury in rats.Objective: To investigate whether ginsenoside Rg1 can alleviate inflammation by regulating microglial/macrophage polarization.Methods: The study included in vivo and in vitro experiments.In vivo experiment: 60 female SD rats were selected and moderate spinal cord injury model was established by modified Allen method.The experiment was divided into four groups: blank control group,blank control group + ginsenoside Rg1 intervention group,spinal cord injury modeling group,spinal cord injury + ginsenoside Rg1 intervention group.At different time points,the spinal cord was sectioning and paraffin embedded.Total RNA and total protein were extracted and Western Blot and RT-q PCR were used to detect pro-inflammatory factors(TNF-a and IL-6),anti-inflammatory factors(IL-4 and IL-10)and macrophage/microglia polarization markers,or immunohistochemical detection.In vitro experiment: The primary spinal microglia of rats were cultured and divided into four groups: control group,ginsenoside Rg1 group,LPS + IFN-γ stimulation group,ginsenoside Rg1 + LPS + IFN-γstimulation group.Total RNA and total protein were extracted and detected by Western Blot and RT-q PCR for pro-inflammatory factors(TNF-a and IL-6),anti-inflammatory factors(IL-4 and IL-10)and macrophage/microglia polarization markers,or cell immunofluorescence assay.Results: Ginsenoside Rg1 could inhibit the expression of pro-inflammatory factors TNF-a and IL-6,promote the expression of anti-inflammatory factors IL-4 and IL-10,and inhibit the M1 polarization of macrophage/microglia.Conclusion: Ginsenoside Rg1 can reduce inflammation by regulating the polarization of macrophages / microglia. |
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