LncRNA-SNHG15 Regulation Proliferation,Apoptosis And Invasion Of Bladder Cancer Cell

Bladder cancer is one of the most common tumors in the urinary system.It is easy to relapse,progress and metastasize,because it is become the most attracted attention in urological tumor research^[1].Elucidating the key molecular mechanisms of bladder cancer invasion and metastasis will not only provide a theoretical basis for inhibiting bladder cancer invasion or metastasis,but also provide new perspectives and ideas for bladder cancer prognosis evaluation,early diagnosis and targeted therapy.Long non-coding RNA refers to non-coding RNA subclasses with transcripts longer than 200nucleotides.They participate in the regulation of protein encoding genes at different levels（epigenetic regulation,transcriptional regulation and post-transcriptional regulation）in the form of RNA..Recently,many studies have shown that Long non-coding RNA has abnormally expressed in bladder cancer,kidney cancer,prostate cancer,breast cancer,liver cancer,lung cancer and other tumors,further more regulates the expression level of downstream target genes,so as to play an important role in tumorigenesis,development and metastasis^[2].The current research on the expression level and mechanism of Long non-coding RNA-SNHG15 in bladder cancer is still blank.This study uses modern molecular biology techniques to investigate the expression of Long non-coding RNA-SNHG15 in bladder cancer tissues and cell lines and its role in regulating bladder cancer cells,in an attempt to reveal the mechanism of Long non-coding RNA-SNHG15 in the proliferation,apoptosis and invasion of bladder cancer cells.The results showed that long-chain non-coding RNA-SNHG15 was highly expressed in bladder cancer tissues and cells,and was related to tumor size,stage,and metastasis,while s i RNA could inhibit the expression of Long non-coding RNA-SNHG15.Further research found that down-regulating the expression of Long non-coding RNA-SNHG15 in bladder cancer cells can inhibit the EMT process of bladder cancer cells,reduce the ability of bladder cancer cells to invade,and play an important role in regulating the invasion of bladder cancer cells.This study is divided into three parts;The first part:The Expression level of lncRNA-SNHG15 in bladder cancer tissue.Objective:To study the expression of Long non-coding RNA-SNHG15 in bladder cancer tissue.Method:The expression of Long non-coding RNA-SNHG15 in bladder cancer was detected by RT-PCR.（Graph pad 8.0.1.224）software was used for statistical analysis.,The t-test is used for the comparison of two sample mean.The difference was statistically significant（P<0.05）.Results:Compared with the normal tissues,Long non-coding RNA-SNHG15 was highly expressed in bladder cancer tissues as shown in Figure 1 Long non-coding RNA-SNHG15 was not related to gender（P=0.999）and age（P=0.3268）,but was related to tumor size（P=0.0465）and metastatic of carcinoma（P=0.015）.Tab 1Conclusion:The Expression of long-chain non coding RNA is high in bladder cancer tissue compare to the normal tissue.Lcrna-snhg15 was not related to gender and age,but was related to tumor size and metastatic of carcinoma Tab 1.The second part:lncRNA-SNHG15 regulates the proliferation and apoptosis of bladder cancer cells;Objective:To study the expression of long-chain noncoding RNA in bladder cancer cells and the effect of LncRNA-SNHG15 on proliferation and apoptosis.Methods:bladder cancer cell lines（UMUC3,5637,J82、T24）and normal human bladder cell lines（sv-huc-1）were cultured in vitro;Bladder cancer cells and normal human bladder cells（sv-huc-1）were transfected with small interfering RNA（siRNA）,which were divided into si-Long non-coding RNA-SNHG15 positive group and Si-NC negative control group;the expression of lncrna-snhg15 in bladder cancer cells were detected by real-time PCR and Cell Counting Kit-8 assay was utilized to assess the impact of Long non-coding RNA-SNHG15 on the proliferation of bladder cancer cells.All the experimental results were repeated three times,and statistical analysis was carried out by（Graph pad 8.0.1.224）software.T-test was used for comparison between two groups;the difference was statistically significant（P<0.05）.Results:compared with the normal bladder cells,the expression of lncrna-snhg15 in bladder cancer cells was higher than normal bladder cell（P<0.05）,as shown in the Figure 2;after siRNA transfection,the expression of lncrna-snhg15 in bladder cancer cells was significantly down regulated（P<0.05）,as shown in the Figure 3a,b,meanwhile,the proliferation of bladder cancer cells was inhibited and apoptosis was increased.Conclusion:The results showing that lncRNA-SNHG15 is highly expressed in bladder cancer cells,siRNA can inhibit the expression of lncRNA-SNHG15;by down regulating the expression of lncRNA-SNHG15,it can inhibit the proliferation of bladder cancer cells and promote the apoptosis of bladder cancer cells.The third part:Lncr RNA-SNHG15 Regulating the aggressiveness of bladder cancer cells.OBJECTIVE:To assess the connection between the expression of Lncr RNA-SNHG15in bladder cancer cells and epithelial-mesenchymal transition（EMT）of bladder cancer cells,and investigate the impact of Lncr RNA-SNHG15 on the invasion ability of bladder cancer cells.Method:In vitro culture of bladder cancer UMUC3,T24 cell line and typical human bladder cells,SV-HUC-1;bladder cancer cells and normal human bladder cells SV-HUC-1 were transfected with siRNA,and the invasion ability of bladder cancer cells was identified by Transwell assay.Results:Compared with the normal control group,the expression of Lncr RNA-SNHG15in bladder cancer cells after siRNA transfection was down-regulated,and the invasion ability of bladder cancer cells was significantly inhibited P<0.05 as shown in the Figure4.Conclusion:Down-regulating the expression of lncrna-snhg15 in bladder cancer cells can inhibit the EMT process of bladder cancer cells,reduce the invasion ability of bladder cancer cells,and play an important role in regulating the invasion of bladder cancer cells.This suggests that lncrna-snhg15 is a cancer-promoting gene,which can provide a potential molecular targeting marker for future bladder tumor targeted therapy.